氧气在Baijiu堆叠过程中影响空间异质性和微生物继承动态
Liangqiang Chen1, Xing Qin2, Guozheng Wang2
1Laboratory of Brewing Microbiology and Applied Enzymology, Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, Jiangsu, China; Moutai Institute, Renhuai 564500, Guizhou, China; Guizhou Key Laboratory of Microbial Resources Exploration in Fermentation industry, Kweichow Moutai Group, Renhuai 564500, Guizhou, China.
Bioresource technology
|May 18, 2024
概括
自发固态堆叠过程 (SSSP) 在Baijiu发酵中产生了明显的氧层. 乙杆菌 (Acetilactobacillus jinshanensis) 在低氧区繁荣发展,维持微生物群体结构并提高发酵效率.
科学领域:
- 微生物学 微生物学
- 发酵科学 发酵科学
- 环境生物技术 环境生物技术
背景情况:
- 自发固态堆叠过程 (SSSP) 对于Baijiu发酵至关重要.
- 了解微生物组合和空间异质性是优化发酵效率的关键.
- 在SSSP过程中,环境微生物得到了丰富和组合.
研究的目的:
- 研究SSSP如何在堆叠堆中创造空间异质性.
- 确定驱动这种异质性的微生物动力学和环境因素.
- 了解氧气梯度和占主导地位的微生物物种在塑造SSSP微生物群中的作用.
主要方法:
- 堆叠堆的时间空间采样.
- 对氧度梯度的分析,以定义不同的层 (枯竭,过渡,丰富).
- 微生物社区分析,重点关注主导物种及其代谢作用 (例如,poxL,catE基因表达).
主要成果:
- 基于氧气交换难度,SSSP建立了不同的氧气定义层.
- 在这些层中,微生物的继承率显著不同 (V枯竭 > V过渡 > V丰富),加速了空间异质性.
- 乙杆菌 (Acetilactobacillus jinshanensis) 主导着低氧层,积极减少氧气干扰并维持异质性.
结论:
- 氧气控制是SSSP期间塑造空间异质性的关键因素.
- 这种异质性导致了特定的功能性微生物组的发展.
- 实施空间异质性管理可以实现对固态发酵系统的精确控制.
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