简短的沟通:存储时间和温度会影响现场采集的血液样本中的血透度值
Nemo E Buchmiller1, Savannah J Weaver1, Robin E Bedard1
1Department of Biological Sciences, California Polytechnic State University, San Luis Obispo, CA 93407-0411, United States of America.
概括
存储野生动物血样本用于度分析需要小心. 冷样本比冷样本的度增加更大,这表明短期冷藏 (≤3天) 是准确的水分评估的最佳选择.
科学领域:
- 环境生理学环境生理学
- 野生动物生物学 野生动物生物学
- 生态研究生态研究
背景情况:
- 气候变化影响息地水文,需要了解动物水生理学.
- 血度是一个关键的水合指标,但样品存储效应未得到充分研究,特别是在现场环境中.
研究的目的:
- 为了研究不同储存条件下的野生蛇样本中的血度的稳定性.
- 为了确定时间和温度对等离子体度完整性的影响,用于生态研究.
主要方法:
- 蛇血样本被储存在塑料管中,温度为2°C (冷藏) 和-18°C (冷).
- 样品在0,2,3,7和29天后进行了度分析.
- 对度的变化与基线值和温度处理之间进行了比较.
主要成果:
- 随着时间的推移,在制冷和结温度下,等离子体度显著增加.
- 与假设相反,冷样本在7天后比冷样本的度增加更大.
- 储存7天后,度值明显偏离了基线.
结论:
- 长时间储存,特别是冷,可以改变野生动物样本中的血度读数.
- 为了在实地研究中准确地评估水分,将血样本冷藏3天或更短时间.
- 需要进一步的研究,以了解在血储存期间发生的度变化背后的机制.
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