相关实验视频

Updated: Jun 26, 2025

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Published on: January 30, 2014

SENP1通过wnt/β-catenin通路调节间歇性缺氧诱导的微质介导炎症和认知功能障碍

Xiwen Ou¹, Hongwei Wang¹, Yubin Shen¹

¹Department of Respiratory Medicine and Sleep Lab, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China.

European journal of pharmacology

|May 18, 2024

概括

阻塞性睡眠呼吸暂停会导致大脑炎症和认知能力下降. 通过SENP1准β-catenin SUMOylation,可以预防这种神经炎症并改善认知功能.

科学领域:

神经科学是一个神经科学.
睡眠医学睡眠医学
分子生物学分子生物学

背景情况:

阻塞性睡眠呼吸暂停综合征 (OSAS) 与由于间歇性缺氧 (IH) 诱导的神经炎症导致的认知障碍有关.
Wnt信号通路与中枢神经系统疾病有关,但其在OSAS相关的神经炎症中的作用尚不清楚.
SUMOylation参与神经炎症,并可能调节Wnt/β-catenin通路成员.

研究的目的:

调查SUMOylation在IH诱导的神经炎症和神经元损伤中的Wnt/β-catenin通路上的调节机制.
探索向SUMOylation治疗OSAS认知功能障碍的潜力.

主要方法:

进行了体外和体内分子生物学实验.
这项研究研究了IH对β-catenin SUMOylation,神经炎症和神经元损伤的影响.
研究了SENP1在调节β-catenin de-SUMOylation和Wnt/β-catenin通路激活中的作用.

主要成果:

IH诱导β-catenin SUMOylation,导致微质介导的炎症和神经元损伤.
SENP1促进了β-catenin的脱SUMOylation,从而激活了Wnt/β-catenin通路.
在暴露于IH的小鼠中,SENP1激活Wnt/β-catenin通路可缓解神经炎症和神经元损伤.
这种干预措施改善了与IH相关疾病的小鼠的认知功能障碍.

结论:

由IH引起的神经炎症和神经元损伤是由β-catenin SUMOylation介导的.
通过SENP1调节β-catenin的de-SUMOylation和随后的Wnt/β-catenin通路激活可以减轻这些影响.
针对β-catenin的SUMOylation呈现出与OSAS相关的认知功能障碍的潜在治疗策略.

关键词:

认知功能障碍认知功能障碍间歇性缺氧间歇性缺氧神经炎症是一种神经炎症.这就是SENP1的意义.在Wnt/β-catenin信号传输中.

更多相关视频

Evaluation of Synapse Density in Hippocampal Rodent Brain Slices

Evaluation of Synapse Density in Hippocampal Rodent Brain Slices

Published on: October 6, 2017

Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation

Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation

Published on: February 16, 2018

相关概念视频

Canonical Wnt Signaling Pathway

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...