相关实验视频

Updated: Jun 26, 2025

Introducing a Gene Knockout Directly Into the Amastigote Stage of Trypanosoma cruzi Using the CRISPR/Cas9 System
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Introducing a Gene Knockout Directly Into the Amastigote Stage of Trypanosoma cruzi Using the CRISPR/Cas9 System

Published on: July 31, 2019

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分子工具来调节Trypanosoma cruzi中的基因表达

Lays Adrianne M Trajano-Silva1, Simon Ngao Mule1, Giuseppe Palmisano2

  • 1Glycoproteomic Laboratory, Parasitology Department, Institute of Biomedical Science II, University of Sao Paulo, Sao Paulo, Brazil.

Advances in clinical chemistry
|May 18, 2024
PubMed
概括

由于特有的寄生虫生物学,Trypanosoma cruzi的遗传操纵很困难. 聚类正规间隔的短巴林德罗姆重复Cas9相关系统为研究查加斯病寄生虫提供了新的工具.

关键词:
这就是CRISPR/Cas9的作用.遗传工具 是一种遗传工具.基因组编辑 基因组编辑试osoma cruzi 是一种类型的虫.

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Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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