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预adipocytes在瘤微环境中增强了黑色素瘤进展和M2巨细胞两极分化

Tae Jin Jeon¹, Ok-Hyeon Kim², Hana Kang¹

¹Department of Global Innovative Drugs, Graduate School of Chung-Ang University, Seoul, 06974, South Korea.

Biochemical and biophysical research communications

|May 19, 2024

概括

前脂细胞是一种脂肪细胞,可能会恶化黑色素瘤的生长和传播,通过外体细胞与癌细胞进行通信. 抑制外体减少了黑色素瘤的进展和转移相关的标志物.

科学领域:

在瘤学瘤学.
细胞生物学细胞生物学
癌症研究癌症研究

背景情况:

黑色素瘤是一种严重的皮肤癌,由黑色素细胞引起.
预脂细胞在黑色素瘤进展中的作用尚不清楚.

研究的目的:

研究前脂肪细胞对黑色素瘤细胞增殖,生长和转移的影响.
探索黑色素瘤细胞和前脂肪细胞之间的相互作用机制,特别是外体细胞介导的通信.

主要方法:

共同培养小鼠黑色素瘤B16细胞与3T3L1前脂质细胞,形成混合球形.
分析转移相关蛋白质 (YAP,TAZ,PD-L1) 和microRNA-155 (miR-155) 的表达.
使用外体抑制剂GW4869来评估外体在细胞通信和黑色素瘤进展中的作用.

主要成果:

与只有黑色素瘤的球体相比,混合球体显示出黑色素瘤扩散和生长的增加.
转移标志物 (YAP,TAZ,PD-L1) 和miR-155在混合球体中升高.
治疗GW4869抑制了黑色素瘤的生长,减少了转移标志物和miR-155,减少了巨细胞M2标志物诱导,表明了外体交叉.

结论:

预adipocytes可以通过外因子介导的交叉声调节来增强黑色素瘤的进展和转移.
外体在调解前脂肪细胞对黑色素瘤的前瘤性影响方面发挥着至关重要的作用.
向外体通信可能是黑色素瘤的潜在治疗策略.

关键词:

黑色素瘤是一种黑色素瘤.预adipocytes 是一个细胞.瘤微环境是一个微环境.在 miR-155 系统中,它是 miR-155 的.

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