[a]烯,PCB153和性激素对人类ESC衍生的甲状腺毛囊的影响使用单细胞转录组学
Marta Nazzari1, Mírian Romitti2, Anna M Kip3
1Department of Toxicogenomics, GROW School for Oncology and Developmental Biology, Maastricht University, 6229 ER Maastricht, the Netherlands.
Environment international
|May 19, 2024
概括
内分泌干扰物,如子[a]皮林,根据性激素对甲状腺细胞的影响不同. 这项研究使用人类甲状腺器官来揭示这些对环境毒素的性别特异反应.
科学领域:
- 内分泌学和环境健康
- 毒理学 毒理学 毒理学
- 基因组学就是基因组学.
背景情况:
- 内分泌干扰剂会给环境带来风险,影响甲状腺功能.
- 人类的性别差异影响了对甲状腺疾病的易感性.
研究的目的:
- 研究性激素如何调节甲状腺器官对内分泌干扰物的反应.
- 在不同的荷尔蒙条件下分析对[a]pyrene和PCB153的反应中的转录组变化.
主要方法:
- 人类胚胎干细胞衍生的甲状腺器官被暴露在男性或女性激素配置文件中.
- 器官体随后用子[a]烯或PCB153.3处理.
- 进行了单细胞RNA测序和差异基因表达分析.
主要成果:
- [a]烯改变了甲状腺基因表达,影响了核糖体和氧化酸化基因.
- 两种荷尔蒙混合物和本佐[a]皮林都提高了脂质代谢基因的调节;男性荷尔蒙与本佐[a]皮林相结合导致炎症.
- 在女性荷尔蒙的背景下,[a]皮林对排毒基因 (CYP1A1,CYP1B1,NQO1) 的上调更为强烈. PCB153显示出最小的影响.
结论:
- 单细胞转录组学有效地揭示了内分泌干扰物的体外影响.
- 激素背景显著影响甲状腺对环境毒素的反应.
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