糖原β颗粒表面的特点是,在β-氨基溶解之前和之后,大小排除色谱和烯排外体光的组合
Damin Kim1, Sanjay Patel1, Jean Duhamel1
1Institute for Polymer Research, Waterloo Institute of Nanotechnology, Department of Chemistry, University of Waterloo, Waterloo, ON N2L 3G1, Canada.
Carbohydrate polymers
|May 19, 2024
概括
糖原颗粒有一个密集的内部和松散的外层,挑战了以前的模型. 这一发现得到了尺寸排除色谱和烯排外体形成的支持,影响了对糖原代谢和粒子组装的理解.
科学领域:
- 生物化学 生物化学
- 生物物理学的生物物理.
- 糖原的新陈代谢过程
背景情况:
- 讨论了糖原结构和可访问性,模型提出了密集或多孔粒子外围.
- 了解糖原颗粒密度对于阐明糖原代谢和蛋白质相互作用的机制至关重要.
研究的目的:
- 为了确定糖原颗粒的局部密度概况.
- 为了调查糖原粒子外围是否密集 (级别模型) 或松散 (吉尔伯特模型).
- 评估β-氨酶处理对糖原颗粒密度的影响.
主要方法:
- 大小排除色谱 (SEC) 用于分析颗粒大小和密度.
- 使用1-pyrenebutyric 酸探测局部密度的烯突变分子形成 (PEF).
- 用β-氨酶 (Gly-β-LD) 对糖原进行处理,以研究结构变化.
主要成果:
- SEC显示不变的内部密度,无论粒子大小和β-氨基解.
- PEF实验显示,糖原颗粒具有密集的内部和松散的冠状.
- 贝塔氨酶治疗没有改变密度概况.
结论:
- 实验结果支持吉尔伯特模型,表明宽松的外围而不是密集的屏障.
- 密集的内部和松散的冠状结构对蛋白质获取和糖原代谢有影响.
- 这种结构影响了糖原β粒子与更大的α粒子的结合.
相关概念视频
Chemistry of Carbohydrates
Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...
Protein and Protein Structure
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme can...
A protein's shape is critical to its function. For example, an enzyme can...
Membrane Carbohydrates
The plasma membrane is a dynamic barrier composed of lipids, proteins, and carbohydrates. It is the epicenter of many cellular processes required for cell growth and survival. Carbohydrates have unique structural and chemical properties that help the plasma membrane to carry out its functions effectively.
Membrane carbohydrates do not have any hydrophobic region and are exclusively located on the cell's outer surface. The addition of sugar molecules or glycosylation of proteins happens in...
Membrane carbohydrates do not have any hydrophobic region and are exclusively located on the cell's outer surface. The addition of sugar molecules or glycosylation of proteins happens in...
Oligosaccharide Assembly
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Multiple sugar molecules that may or may...
Protein Folding Quality Check in the RER
ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...
X-ray Diffraction of Biological Samples
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are scattered by the electron clouds around the sample atoms. The X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal crystal...
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are scattered by the electron clouds around the sample atoms. The X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal crystal...


