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由复杂的微生物群触发的恒常性T助手17细胞反应在体外肠道组织切片中维持

Valerie Beneke^1,2,3, Klaudia M Grieger^1,2,3, Christina Hartwig^3,4

¹Division of Preclinical Pharmacology and Toxicology, Fraunhofer Institute for Toxicology and Experimental Medicine, Hannover, Germany.

European journal of immunology

|May 19, 2024

概括

肠道微生物群中的细分丝状细菌 (SFB) 显著增强了肠道组织中的T辅助细胞17 (Th17) 反应和相关介质. 这一发现为研究肠道炎症和开发治疗方法提供了新的途径.

科学领域:

免疫学免疫学免疫学
微生物学微生物学
胃肠病学胃肠病学

背景情况:

分段丝状细菌 (SFB) 是影响免疫成熟和炎症的肠道共生体.
像克罗恩病这样的慢性疾病与肠道微生物组的改变有关.
初级肠道组织切片为研究宿主-微生物相互作用提供了一个ex vivo模型.

研究的目的:

调查SFB殖民化是否在肠道组织中增强T助手17 (Th17) 细胞反应.
评估用于微生物组研究的初级肠道组织切片的有用性.

主要方法:

来自SFB阳性和SFB阴性小鼠的初级小肠组织切片的制备.
使用抗CD3/CD28或康卡纳瓦林A的组织切片的ex vivo刺激.
分析微生物组合,Th17细胞频率和相关介质 (SAA,IgA).

主要成果:

阳性和阴性SFB小鼠表现出不同的微生物组形状和Th17细胞水平.
SFB存在与Th17细胞介导体 (SAA,IgA) 活体增加15倍相关.
观察到的表型可以通过共同住房转移,这表明微生物的影响.

结论:

原生肠道组织切片有效地反映了肠道微生物引起的免疫变化.
SFB显著促进了肠道中与Th17细胞相关的反应.
这种ex vivo模型对于疾病建模和肠道健康的治疗评估有价值.

关键词:

免疫媒介疾病免疫媒介疾病肠道组织切片肠道组织切片微生物群中的微生物群这是SFB的SFB.Th17 这是一个很好的方法.

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