Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

In Vivo Monitoring of Thrombo-Inflammatory Biomarkers via Molecularly Imprinted Polymer-Integrated Hydrogel Microneedles.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Ultra-rapid nanoplasmonic colorimetry in microfluidics for antimicrobial susceptibility testing directly from specimens.

Nature nanotechnology·2026
Same author

Machine-Learning-Aided Advanced Electrochemical Biosensors.

Advanced materials (Deerfield Beach, Fla.)·2025
Same author

Elderly Engagement with Technology for Healthcare: A Survey-Based Study.

Studies in health technology and informatics·2025
Same author

Publisher Correction: Next-generation rapid phenotypic antimicrobial susceptibility testing.

Nature communications·2025
Same author

Next-generation rapid phenotypic antimicrobial susceptibility testing.

Nature communications·2024

相关实验视频

Updated: May 13, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.2K

在电化学生物传感应用中以表面为基础的多重体适应器生成和生物功能化.

Seyed Vahid Hamidi1, Arash Khorrami Jahromi1, Imman I Hosseini1

  • 1Department of Bioengineering, McGill University, Montreal, Quebec, H3A 0E9, Canada.

Angewandte Chemie (International ed. in English)
|May 20, 2024
PubMed
概括
此摘要是机器生成的。

通过表面滚动圆放大 (RCA) 生成的多重体吸收体显著提高了生物传感器的灵敏度和亲和力. 这种新的方法为检测像SARS-CoV-2尖端蛋白这样的目标增加了10倍的亲和力和4倍的灵敏度.

关键词:
电化学适应感应传感器多种多重体的体体.纳米/微岛是一个小岛.房间温度滚动圆放大器的放大器

更多相关视频

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

9.8K
Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

1.4K

相关实验视频

Last Updated: May 13, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.2K
Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
09:33

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

9.8K
Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

1.4K

科学领域:

  • 生物技术是生物技术.
  • 纳米技术 纳米技术
  • 生物传感器工程学

背景情况:

  • 由于具有多个结合位,多聚合的阿普坦与单聚合的形式相比,具有更高的结合亲和力.
  • 开发敏感和选择性的生物传感器仍然是分析物检测的关键挑战.

研究的目的:

  • 通过使用室温滚动圆放大 (RCA) 来设计基于表面的多重模拟器.
  • 开发一种高度敏感和选择性的电化学适应传感器,用于目标分析物检测.
  • 调查表面结构对感应传感器性能的影响.

主要方法:

  • 通过使用化学修饰的原料,通过RCA生成基于表面的多分子体.
  • 复杂化多重体的aptamers到间距开启器,以接近传感表面.
  • 表面放大过程的表征和放大时间的优化.
  • 使用平面和纳米/微岛 (NMI) 工作电极 (WE) 的电化学检测.
  • 使用SARS-CoV-2尖端蛋白 (SP) 和临床唾液样本进行测试.

主要成果:

  • 多聚合物适应传感器比单聚合物适应传感器具有超过10倍的亲和力和近4倍的灵敏度.
  • 纳米/微岛 (NMI) WEs显著提高了缓冲和唾液中的敏感性.
  • 使用NMI多重体感应传感器实现了低于2 fg/mL的检测极限 (LOD).
  • 开发的口味传感器通过患者的唾液样本成功验证.

结论:

  • 通过RCA通过表面生成的多重合体吸收体提供了一种强大的策略,用于提高吸收传感器性能.
  • 基于NMI的电极结构与多重体体相结合,为生物传感应用提供了更高的灵敏度和选择性.
  • 开发的电化学适应传感器证明了在复杂的生物介质中检测生物标志物的临床潜力.