微流体传递系统和温度强烈影响过渡传递的效率
Michaela Dehne1,2, Simon Valentin Neidinger3, Michael Stark3
1Institute of Technical Chemistry, Leibniz University Hannover, Hannover 30167, Germany.
ACS omega
|May 20, 2024
概括
一个新的3D打印的微流体系统提高了CHO-K1细胞中短暂转染 (TTF) 的效率. 在22°C达到最佳效果,将传染效率与细胞膜相位过渡相关联.
科学领域:
- 生物技术是生物技术.
- 细胞生物学 细胞生物学
- 微流体学 微流体学
背景情况:
- 暂时转染 (TTF) 对于基因表达研究至关重要.
- 有效的多重复合体吸收和基因表达取决于优化TTF条件.
- 在生物制药研究中,CHO-K1细胞被广泛使用.
研究的目的:
- 开发和评估3D打印的微流体系统,用于CHO-K1细胞的直接TTF.
- 研究传染后化时间和温度对传染效率,活力和可活细胞度的影响.
- 探索细胞膜相位过渡和转染结果之间的关系.
主要方法:
- 使用3D打印的微流体装置直接TTF悬浮CHO-K1细胞.
- 系统地改变传染后的化时间 (t) 和温度 (T).
- 测量了传染效率 (TFE),活力 (V) 和可活细胞度 (VCC).
主要成果:
- 微流体系统的性能远远超过了手动传染方法.
- 在t = 1小时和T = 22°C时观察到最佳的传染输出 (VCC × TFE).
- 传染效率在19°C左右显著增加,与细胞膜相位过渡温度一致.
结论:
- 3D打印的微流体系统为CHO-K1细胞中的TTF提供了一种优越的方法.
- 传染后的化温度显著影响传染效率和细胞活力.
- 与相变相关的细胞膜顺序和透性,在确定转染效率方面发挥着关键作用.
相关概念视频
Mechanisms of Heat Transfer I
Just as interesting as the effects of heat transfer on a system are the methods by which the heat transfer occur. Whenever there is a temperature difference, heat transfer occurs. It may occur rapidly, such as through a cooking pan, or slowly, such as through the walls of a picnic ice box. So many processes involve heat transfer that it is hard to imagine a situation where no heat transfer occurs. Yet, every heat transfer takes place by only three methods: conduction, convection, and radiation.
Mechanisms of Heat Transfer II
In convection, thermal energy is carried by the large-scale flow of matter. Ocean currents and large-scale atmospheric circulation, which result from the buoyancy of warm air and water, transfer hot air from the tropics toward the poles and cold air from the poles toward the tropics. The Earth’s rotation interacts with those flows, causing the observed eastward flow of air in the temperate zones. Convection dominates heat transfer by air, and the amount of available space for the airflow...
Joule-Thomson Effect
The Joule-Thomson effect, also known as the Joule-Kelvin effect, describes the temperature change of a fluid when it is forced through a valve or porous plug while keeping it in a thermally insulated environment. This experiment is called a throttling process. This is an important effect widely used in refrigeration and the liquefaction of gases.
This experiment forces high-pressure gas through a throttle valve or a porous plug to a lower-pressure region. The gas expands as it passes through to...
This experiment forces high-pressure gas through a throttle valve or a porous plug to a lower-pressure region. The gas expands as it passes through to...
Laminar Flow
Laminar flow represents a smooth, orderly fluid motion where particles move along parallel paths, resulting in minimal mixing between layers. Streamlined particle paths characterize this flow regime and occur under conditions where viscous forces dominate over inertial forces. The distinction between laminar, transitional, and turbulent flow is primarily determined by the Reynolds number, a dimensionless quantity calculated as:
Turbulent Flow: Problem Solving
Carbonation is a process used to dissolve carbon dioxide gas in a liquid, commonly used in the production of carbonated beverages. Achieving efficient carbonation requires careful control of temperature, pressure, and flow conditions. By adjusting these parameters, carbonation efficiency can be maximized, producing a higher concentration of CO2 in the liquid.
Temperature is a key factor in CO2 solubility. In this case, the CO2 gas and the liquid are cooled to 20°C. Lower temperatures enhance...
Temperature is a key factor in CO2 solubility. In this case, the CO2 gas and the liquid are cooled to 20°C. Lower temperatures enhance...
Minor Losses in Pipes
In pipe systems, minor losses refer to energy losses arising from components such as valves, bends, fittings, expansions, and other features that disrupt the steady flow of fluid. These disturbances cause energy dissipation through turbulence and resistance, which engineers quantify to manage system efficiency effectively.
Valves play a significant role in generating minor losses by obstructing or redirecting the fluid flow. When a valve is closed or partially closed, it restricts the flow...
Valves play a significant role in generating minor losses by obstructing or redirecting the fluid flow. When a valve is closed or partially closed, it restricts the flow...


