对Limosilactobacillus fermentum菌体的表型特征和基因组分析
Can Zhang1,2,3, Xingyu Quan1,2,3, Weiqi Lian1,2,3
1Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, 010018, PR China.
Current research in food science
|May 20, 2024
概括
从Limosilactobacillus fermentum中分离出了一个新的lysogenic菌体LFP03. 这种菌体特性为L. fermentum菌体基因组学和工业应用的稳定性提供了洞察力.
科学领域:
- 微生物学 微生物学
- 细菌学 细菌学是一门学科.
- 病毒学 病毒学
背景情况:
- Limosilactobacillus fermentum因其有益的特性而被广泛使用.
- 溶解性菌体可以溶解L. fermentum,造成经济损失.
研究的目的:
- 为了描述一种从L. fermentum.中分离出来的新型溶解性菌体LFP03.
- 了解LFP03的基因组和生物特性,用于菌体控制策略.
主要方法:
- 从L. fermentumIMAU 32510中诱导lysogenic菌体LFP03,使用紫外线照射.
- 用电子显微镜进行菌体分类 (Caudoviricetes).
- 基因组测序和分析 (39556个基因组,46.08%的GC,20个功能蛋白).
- 生物分析包括感染的最佳多重性,爆发大小,温度和pH稳定性以及吸附动力学.
主要成果:
- 与其他L. fermentum菌体相比,菌体LFP03基因组表现出独特的删除,反转和转位.
- 感染的最佳多重性为0.1,爆发大小为133.5±4.9 PFU/感染细胞.
- 体LFP03对温度 (48.98%的存活率在50°C) 和pH (在pH2处失活) 显示出敏感性,但对吸附的影响很小.
- 二元酸盐加速吸附,而氨基醇几乎没有影响.
结论:
- 对LFP03的表征扩大了对L. fermentum菌体的知识.
- 这些发现为改善产品稳定性和开发L. fermentum应用中的菌体控制措施提供了理论基础.
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