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缺乏功能性的CCA尾巴的全长tRNA被选择性地排序到细胞外囊泡的光层中
Chantal Scheepbouwer1,2,3, Ernesto Aparicio-Puerta4, Cristina Gómez-Martin3,5
1Department of Neurosurgery, Cancer Center Amsterdam, Amsterdam University Medical Center, VU University, Amsterdam, Netherlands.
哺乳动物细胞分泌的转移RNAs (tRNAs) 缺乏一个功能性的3' CCA尾巴在小细胞外囊泡 (sEVs). 这表明了SEV中细胞通信和货物选择的新机制.
科学领域:
- 细胞外囊泡生物学 细胞外囊泡生物学
- 在RNA生物学,RNA生物学.
- 分子细胞生物学 分子细胞生物学
背景情况:
- 小细胞外囊泡 (sEVs) 是细胞分泌的与脂质结合的粒子,携带各种RNA物种.
- 微RNAs (miRNAs) 是经过充分研究的sEV货物,但结构性非编码RNAs (ncRNAs) 的分类仍未得到充分研究.
- 技术上的局限性阻碍了分析高度结构化的ncRNAs,如SEV中的转移RNA (tRNAs).
研究的目的:
- 在SEV中描述结构化ncRNAs的相对丰度,包括tRNAs,小核子RNAs (snoRNAs) 和YRNAs.
- 调查与EV相关的tRNAs的特征和局部.
- 探索特定的tRNA分类为sEVs的潜在生理作用.
主要方法:
- 适应ALL-tRNAseq协议,用于在散装EV制剂中分析小ncRNA.
- RNAase保护试验以确定与EV相关的tRNAs的局部.
- 对tRNA 3' CCA尾部完整性的分析和与非EV相关颗粒 (NVEPs) 的比较.
主要成果:
- 全长tRNA是细胞培养衍生的和人体血衍生的EV中占主导地位的小型ncRNA物种,数量超过miRNA.
- 与EV相关的tRNA主要位于EV光层内,受到外部RNAse的保护.
- 大多数光线tRNA具有截断的3'CCA尾巴,使它们不适合氨基酸加载,与NVEP中发现的碎片化tRNA不同.
结论:
- 哺乳动物细胞选择性地将具有功能障碍的3' CCA尾巴的全长tRNA排序成SEVs.
- 这种选择性分类表明通过tRNA出口进行细胞间通信的新机制.
- 这些发现为分泌EV的生理作用及其光载载荷选择过程提供了新的视角.
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