翻译后的数字数据编码到哺乳动物细胞群的基因组中
Alec Callisto1, Jonathan Strutz1, Kathleen Leeper2,3
1Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.
bioRxiv : the preprint server for biology
|May 20, 2024
概括
这项研究引入了一种新的基于DNA的生物信号编码器,使用终端脱核样转移酶 (TdT) 来直接将细胞信号转化为基因组DNA数据. 该系统准确地在HEK-293T细胞中编码了"HELLO WORLD",证明了可靠的生物数据存储.
科学领域:
- 分子生物学分子生物学
- 合成生物学 合成生物学
- 基因组学就是基因组学.
背景情况:
- 高分辨率的蜂信号编码对于理解复杂的生物过程至关重要.
- 现有的基于DNA的生物信号编码器依赖信号依赖的基因表达来激活DNA改变酶.
- 直接的酶信号相互作用提供了一个更简单和潜在的更有效的编码机制.
研究的目的:
- 为了展示一个新的生物信号编码系统的概念验证,利用终端脱氧核酸转移酶 (TdT).
- 为了研究细胞信号与TdT用于DNA数据编码的直接相互作用.
- 建立一种存储哺乳动物细胞基因组内的生物信号信息的方法.
主要方法:
- 利用CRISPR-Cas9产生双链DNA断裂,创造3'OH末端作为基质.
- 采用终端脱氧核基转移酶 (TdT),一种模板独立的DNA聚合酶,用于信号依赖的核酸结合.
- 设计了一个系统,将各种生物信号和"HELLO WORLD"信息编码到HEK-293T细胞基因组DNA中.
主要成果:
- 证明了不同生物信号度的成功分辨和编码到基因组DNA中.
- 在HEK-293T细胞中在人口层面编码"HELLO WORLD"消息时实现了91%的准确性.
- 验证了TdT作为响应细胞信号的直接效应分子的功能.
结论:
- 介绍了一个简单的,可设计的系统,用于直接生物信号到DNA编码.
- 确定了在哺乳动物细胞基因组中可靠地存储本地生物信号信息的可行性.
- 它为使用基因组DNA进行先进的细胞数据记录和分析铺平了道路.
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