纯能信号驱动罗塔病毒病理生理学的多个方面
bioRxiv : the preprint server for biology
|May 20, 2024
概括
在小鼠中,P2Y1受体信号加剧了轮状病毒感染,增加了腹和病毒分泌. 用MRS2500阻断这些受体可以减少疾病的严重程度和传播,这表明潜在的治疗点.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- 罗塔病毒感染会导致儿童严重腹,目前的治疗方法在减轻症状方面缺乏有效性.
- purinergic信号传递,特别是ADP激活P2Y1受体,已经涉及到轮状病毒感染的细胞.
- P2Y1受体在体内轮状病毒病原发生中的作用尚不清楚.
研究的目的:
- 用小鼠模型研究P2Y1受体信号在轮状病毒感染中的作用.
- 评估P2Y1受体对抗剂在减少轮状病毒引起的腹和病毒分泌方面的治疗潜力.
主要方法:
- 在C57BL/6J小鼠中感染了轮状病毒株D6/2.2.
- 每天用选择性P2Y1抗剂 (MRS2500) 或盐水进行口服输液治疗.
- 监测腹的严重程度,发病率和病毒分泌 (qPCR).
- 在感染后的第3天和第5天对小肠组织学检查.
主要成果:
- MRS2500治疗显著降低了轮状病毒腹的严重程度,患病率和发病率.
- 抑制P2Y1受体导致从感染后的第四天开始减少病毒分泌.
- MRS2500治疗限制了轮状病毒腹在 littermates之间传播.
结论:
- P2Y1受体信号传递是小鼠轮状病毒感染病变的关键因素.
- 通过像MRS2500这样的抗剂准P2Y1受体显示出作为罗塔病毒抗腹和抗病毒疗法的希望.
- 抑制P2Y1受体可能会减少轮状病毒疾病的负担和传播.
更多相关视频
11:40Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins
Published on: April 17, 2020
8.6K
08:01Author Spotlight: Investigating Viral Disruption of Intestinal Epithelial Signaling – Research Insights and Future Directions
Published on: January 19, 2024
1.3K
相关概念视频
GPCRs Regulate Adenylyl Cylase Activity
5.5K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
5.5K
Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
Rous Sarcoma Virus (RSV) and Cancer
5.1K
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
5.1K
Pathophysiology of Peptic Ulcer Disease: Injurious Factors
570
Peptic ulcers are sores on the stomach's inner lining and the upper small intestine, which are the result of disruptions in the mucosal layer that houses parietal cells which produce gastric acid, and chief cells which secrete pepsinogen.
In the antrum region, G cells secrete the gastrin hormone that binds to gastrin-cholecystokinin-B (CCK2) receptors on parietal and enterochromaffin-like (ECL) cells in the fundic glands. Simultaneously, the vagus nerve releases acetylcholine, which binds...
In the antrum region, G cells secrete the gastrin hormone that binds to gastrin-cholecystokinin-B (CCK2) receptors on parietal and enterochromaffin-like (ECL) cells in the fundic glands. Simultaneously, the vagus nerve releases acetylcholine, which binds...
570
Mechanisms of Retrovirus-induced Cancers
5.1K
Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
5.1K
Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.1K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K
