来自单细胞的超强增强体互动体链接集群和转录
Derek J Le1,2,3,4, Antonina Hafner1,4, Sadhana Gaddam3
1Department of Developmental Biology, Stanford University, Stanford, CA, United States.
bioRxiv : the preprint server for biology
|May 20, 2024
概括
超级增强剂 (SE) 很少直接接触,但在单细胞中形成"社区". 这些SE社区影响基因转录突发,挑战目前的基因调节模型.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 基因表达调节涉及增强剂和促进剂,通常是双对研究.
- 先进的基因组折叠技术揭示了超级增强剂 (SE) 之间的多途径相互作用,但缺乏单细胞频率和转录相关性数据.
研究的目的:
- 研究单个哺乳动物细胞中的超强增强剂 (SE) 相互作用的频率和性质.
- 探索SE集群,基因组组织和转录活动之间的关系.
- 挑战关于SE聚类在基因调控中的现有假设.
主要方法:
- 利用多重成像来绘制成千上万种哺乳动物核中的376个超级增强器 (SE) 的3D位置.
- 分析了SE-SE联系,SE"社区"的形成,以及它们与基因组链接的关联,Pol2聚类和核细分.
主要成果:
- 单细胞成像揭示了罕见的SE-SE直接接触,但频繁形成较宽松的SE"社区" (平均4-5个SE).
- SE社区集会是一个受基因组链接,Pol2聚类和核细分影响的合作过程.
- 更大的SE社区与更频繁和更大的转录突发有显著的关联.
结论:
- 较大的SE社区与转录突发的频率和大小增加有关.
- 这些发现挑战了关于SE集群在转录监管中的作用的现有假设.
- 提供了对SE相互作用和基因表达控制的新单细胞见解.
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