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使用纳米孔DRS进行mRNA psi分析,揭示了特定于细胞类型的伪尿化

Caroline A McCormick¹, Michele Meseonznik¹, Yuchen Qiu¹

¹Dept. of Bioengineering, Northeastern University, Boston, MA.

bioRxiv : the preprint server for biology

|May 20, 2024

概括

人类mRNA中的伪尤里丁 (psi) 修饰使用纳米孔测序绘制成全转录组图. 细胞类型影响psi水平,影响基因表达和蛋白质生产.

科学领域:

* 分子生物学 * 分子生物学
* 基因组学是一个学科.
*RNA生物学 *RNA生物学

背景情况:

* 伪尤里丁 (psi) 是一种流行的mRNA修饰,由TRUB1和PUS7.7等酶合成.
*理解psi的作用需要准确的转录组范围的映射和分析其位置占用.

研究的目的:

*为了绘制不同类型的人类细胞中的定位伪尿素占用率.
* 调查psi对基因表达和翻译的影响.
* 为了识别特定于细胞类型的伪尿化模式.

主要方法:

* 利用纳米孔直接RNA测序来进行转录组范围内的psi映射.
*采用Mod-p ID工具来准确识别伪尿素部位,而无需进行化学修饰或cDNA转换.
*比较了来自不同组织类型的六个永生的人类细胞系的位置性psi占用率.

主要成果:

*来自肺部的细胞表现出伪尿素的比例最高,而来自肝脏的细胞表现出最低.
* 在mRNA上保存的伪尿素位点与增加的蛋白质生产相关,这表明它在翻译调节中的作用.
* 在无处不在表达的基因中识别了特定细胞类型的伪尿素修饰.
*具有多个保存或细胞类型特定的伪尿素修饰的特征性转录,包括在同一个动机内.

结论:

* 伪尿素修饰在调节翻译方面发挥着重要作用.
* 细胞类型特定的转基因作用因子是伪尿化模式的关键驱动因素.
*使用Mod-p ID进行纳米孔直接RNA测序,为研究定位psi占用及其功能影响提供了一种敏感的方法.

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