Set2和H3K36以特定环境的方式调节Drosophila雄性X染色体,独立于MSL复杂的传播
Harmony R Salzler1, Vasudha Vandadi1, A Gregory Matera1,2,3,4
1Integrative Program for Biological and Genome Sciences, University of North Carolina, Chapel Hill, NC, USA.
bioRxiv : the preprint server for biology
|May 20, 2024
概括
在Drosophila中,剂量补偿涉及MSL复合体. 这项研究表明Set2和H3K36间接支持这一过程,而不是直接招募MSL3,影响两性基因.
科学领域:
- 表观遗传学和基因调控
- 染色体生物学 染色体生物学
- 德洛索菲拉黑虫 模型系统模型
背景情况:
- 在Drosophila中,剂量补偿 (DC) 通过男性特异性致死性 (MSL) 复合体对雄性X结合基因进行上调.
- 目前的模型表明,MSL复合体的传播是由MSL3与基因素标记的相互作用介导的,特别是Set2依赖的H3K36me3.
- 关于Set2的特定目标,与基因组变异 (H3.2/H3.3) 的潜在冗余性,以及区分男性特异性和X特异性效应,存在不确定性.
研究的目的:
- 为了研究Set2和H3K36三甲基化在Drosophila剂量补偿中的作用.
- 区分H3K36me3在MSL复杂传播和X基因调节中的直接和间接作用.
- 探索潜在的性别特异性与X染色体特异性调节机制.
主要方法:
- 基因组方法在Drosophila突变中用于H3K36 (残留物) 和Set2 (编写器).
- 分析包括基因表达概况和MSL3结合的评估.
- 还检查了H3.2和H3.3基因组变异的突变,以及不同发育阶段和组织的研究.
主要成果:
- Set2在X基因调节中发挥作用,但突变者的表达变化经常反映在女性身上,表明非性别特异性的影响.
- 失去H3K36或Set2导致异质基因表达变化,其中一些基因表现出相反的反应,表明相互的H3K36me功能.
- 组合的H3.2/H3.3突变并没有显示出一致的X基因下调或与MSL3结合的相关性. 在Set2/H3K36调节中,BEAF-32和绝缘体蛋白都涉及到.
结论:
- 这些发现挑战了H3K36me3.3.的直接MSL复杂招聘的主要模式.
- Set2和H3K36可能间接支持剂量补偿,通过MSL使用的,但两性共同的机制.
- 通过Set2和H3K36的调节取决于上下文,并涉及绝缘蛋白.
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