培养水力学对Arthrospira platensis使用一次性光生物反应器系统生产的影响,通过CFD支持的方法
S Furkan Demirden1, Barıs Erdogan1, Deniz Şenyay Öncel2
1Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, Turkey.
Biotechnology progress
|May 20, 2024
概括
生物反应器中较高的机速度显著提高了Arthrospira platensis (Spirulina platensis) 的种植. 230rpm的最佳速度最大限度地提高了干燥生物质,吸收率和叶绿素a,从而提高了体积生产率.
科学领域:
- 生物技术是生物技术.
- 微生物培养 微生物培养
- 生物反应器工程 生物反应器工程
背景情况:
- 藻 (Spirulina platensis) 是一种富含营养的微藻,具有重要的生物技术潜力.
- 优化种植条件,如机速度,对于最大限度地提高生物质产量和生物反应器的生产率至关重要.
研究的目的:
- 为了研究不同机速度对 Arthrospira platensis 的生长和生物化学成分的影响.
- 为了确定在实验室规模的生物反应器中增强Arthrospira platensis种植的最佳机速度.
主要方法:
- 实验是在实验室规模的一次性生物反应器中进行的.
- 计算流体动力学 (CFD) 模拟用于初始机速度的选择.
- 长期培养批量在选定的机速度 (70,130和230rpm) 进行.
- 监测的关键参数包括干生物质重量,吸收值 (AB),甲度和体积生产率.
主要成果:
- 与130和70rpm相比,230rpm的机速度产生了显著更高的干燥生物质重量,吸收率和叶绿素a度.
- 在最初的10天批次中,体积生产率从每分钟70转的0.19±0.02gL-1d-1到每分钟230转的0.39±0.03gL-1d-1不等.
- 在每分钟230转的17天批量中,实现了0.33 ± 0.04 g L-1 d-1.1 的体积生产率.
- 统计分析证实了机速度对培养参数的重要性.
结论:
- 机速度是影响Arthrospira platensis生长和生物反应器生产率的关键因素.
- 建议使用更高的器速度,特别是每分钟230转,以优化Arthrospira platensis的种植,以最大限度地提高生物质和生物化学产量.
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