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长距离表面等离子凝吸收传感器的体外性能,用于在人血清中持续和实时测量万科米辛

Yui Taguchi¹, Koji Toma², Kenta Iitani³

¹Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.

ACS applied materials & interfaces

|May 20, 2024

概括

这项研究开发了一种PMM水凝适应传感器,用于实时监测万科米辛 (VCM). 在未稀释的血清和血液中检测VCM的PMM水凝显示出卓越的性能和稳定性.

科学领域:

生物医学工程生物医学工程
分析化学分析化学
材料科学材料科学材料科学

背景情况:

对于治疗疗效而言,精确监测万科米辛 (VCM) 是至关重要的.
现有的VCM检测方法通常需要样品预处理,并且缺乏实时功能.
传感器的表面修改是提高性能和在复杂的生物矩阵中实现直接检测的关键.

研究的目的:

为了研究表面修饰的水凝对于远程表面等离子体 (LRSP) 适应传感器的适用性.
开发一种传感器,用于实时监测未稀释血清和血液中的万科米辛 (VCM).
为了评估优化的水凝感应传感器的性能和稳定性.

主要方法:

制造具有不同水凝层结构的LRSP传感器芯片,使用聚[2-methacryloyloxyethyl phosphorylcholine (MPC) -co-N-methacryloyl-(L) -tyrosinemethylester (MAT) ] (PMM) 和聚[MPC-co-2-ethylhexyl methacrylate (EHMA) -co-MAT] (PMEM).
通过MAT,将VCM的酸胺基固定到水凝层上.
优化PMM水凝制备和评估传感器性能,稳定性和防腐性质.
在未稀释的血清和血液中应用PMM水凝感应传感器来检测VCM.

主要成果:

与其他传感器配置相比,PMM水凝适应传感器表现出最高的传感器输出和优越的抗物特性.
优化的PMM水凝证明了超过2周的保质期,并允许102天的传感器芯片重复使用,而不会造成显著的性能损失.
在未稀释血清中,PMM水凝达到0.098μM的检测极限和0.18-100μM的动态范围,覆盖治疗范围.
在血清中实现了连续,无冲洗的VCM测量和未稀释血液中的度依赖输出.

结论:

PMM水凝是适合LRSP适应传感器的表面修饰.
开发的PMM水凝适应传感器可以在未稀释的生物流体中对VCM进行敏感,实时和直接的监测.
这项技术具有显著的潜力,可以改善治疗药物监测的vancomycin.

关键词:

一个适应传感器.血液血液血液血液这是一种水凝.在体外 (in vitro) 的情况下.血液中的血清是血清.表面等离子体表面等离子体治疗药物监测治疗药物监测这就是Vancomycin.

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