使用 κ-Carrageenan 亚微凝介质嵌入式生物打印类似组织结构
Hua Zhang1, Tong Zhu2, Yang Luo3
1Research Institute of Smart Medicine and Biological Engineering, Ningbo University; Health Science Center, Ningbo University; Key Laboratory of Precision Medicine for Atherosclerotic Diseases of Zhejiang Province, The First Affiliated Hospital of Ningbo University.
Journal of visualized experiments : JoVE
|May 20, 2024
概括
一种新的 κ-卡拉基南微凝支浴使高质量的嵌入式3D生物打印成为可能. 这种技术支持细胞活力和复杂组织结构制造,用于组织工程应用.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 生物打印技术的技术
背景情况:
- 嵌入式3D生物打印需要专门的水凝支浴来创建生物仿真支架.
- 在优化凝特性以实现精确的生物墨水沉积,细胞活力和构造完整性方面存在挑战.
研究的目的:
- 开发一种新的 κ-卡拉基南子微凝支浴,用于增强嵌入式3D生物打印.
- 为了评估 κ-carrageenan 微凝用于组织制造的材料特性和性能.
主要方法:
- 通过机械研磨制造 κ-卡拉基子微凝.
- 微凝风湿学性质的表征 (宾汉流,剪切稀释).
- 评估凝溶液过渡,自我修复和印刷后去除.
主要成果:
- 产生了具有宾汉流和剪切稀释行为的均质子微凝.
- 微凝浴室促进了生物墨水的顺沉积,并保持了结构完整性.
- 印刷结构显示高细胞活力 (92%) 和强大的细胞增殖.
结论:
- 新型 κ-卡拉基南微凝浴是高质量的嵌入式3D生物打印的有希望的材料.
- 这一战略支持复杂的细胞载体组织结构的开发,用于再生医学.
- 该材料的特性适用于先进的体外组织和器官制造.
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