pH值对蛋白质多糖化合物的乳化性能的影响
Ruyuan Zhang1,2,3, Meinou Corstens2, Zisheng Luo3
1College of Biological Chemical Sciences and Engineering, Jiaxing University, Jiaxing, China.
Journal of the science of food and agriculture
|May 20, 2024
概括
乳清蛋白分离剂-桃糖多糖化合物的表面电荷显著影响了乳液聚合的稳定性. 凝结稳定性通过固态排斥来维持,为创建稳定的食品乳液提供了洞察力.
科学领域:
- 食品科学 食品科学 食品科学
- 合体和表面科学科学
- 材料科学 材料科学 材料科学
背景情况:
- 蛋白质多糖化合物复合物是有效的乳液稳定剂.
- 复杂表面电荷对乳液聚合和凝聚稳定性的影响尚不清楚.
- 研究低电荷界面薄膜在维持乳液凝聚稳定性方面的作用至关重要.
研究的目的:
- 为了研究pH对乳清蛋白分离-桃子多糖化物 (WPI-PGP) 复合物的聚合和凝聚稳定性的影响.
- 了解WPI-PGP复合物的不同表面电荷如何影响乳液稳定性.
- 探索WPI-PGP复合物的潜力,以制备稳定的食品乳液.
主要方法:
- 在不同的pH值 (3,4,5) 下合成WPI-PGP复合物.
- 复杂尺寸和 ζ-潜在的表征.
- 接口流学实验,以评估接口张力和薄膜特性.
- 微流体实验,以评估滴滴稳定与凝聚.
- 长期储存研究,以监测聚合和化.
主要成果:
- 在pH 3,4和5下合成的WPI-PGP复合物表现出不同的大小和z电位 (+2.9, -8.6和-22.8mV,分别).
- 具有pH3的复合体表现出最低的界面张力,并形成最软的膜.
- 所有WPI-PGP复合体都有效地稳定了短时间内对凝聚的液滴.
- 虽然pH值为4和5的乳液在16天内保持稳定,但在pH值为3的8天后发生了广泛的聚合和化,尽管防止了凝聚.
结论:
- 乳液聚合稳定性主要取决于蛋白质多糖化合物的表面电荷.
- 这些乳液的凝结稳定性归因于固体排斥.
- 这些发现为稳定的食品乳液的合理设计提供了宝贵的见解.
相关概念视频
Extraction: Effects of pH
483
Consider a neutral form of an amine, B, with a partition coefficient, K, in a liquid mixture containing organic and aqueous phases. The pH of the aqueous phase affects the charge on acidic and basic solutes, and the charged form is usually more soluble in the aqueous phase. Suppose the conjugate acid form of the amine is soluble only in the aqueous phase while the base form is soluble in both phases. Then the distribution coefficient, D, can be given as the ratio of amine concentration in the...
483
SDS-PAGE
27.8K
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
27.8K
Polyprotic Acids
29.1K
Acids are classified by the number of protons per molecule that they can give up in a reaction. Acids such as HCl, HNO3, and HCN that contain one ionizable hydrogen atom in each molecule are called monoprotic acids. Their reactions with water are:
29.1K
Colloids
17.4K
Children at play often make suspensions such as mixtures of mud and water, flour and water, or a suspension of solid pigments in water known as tempera paint. These suspensions are heterogeneous mixtures composed of relatively large particles that are visible to the naked eye or can be seen with a magnifying glass. They are cloudy, and the suspended particles settle out after mixing. On the other hand, a solution is a homogeneous mixture in which no settling occurs and in which the dissolved...
17.4K
Composition of Polyprotic Acid Solutions as a Function of pH
519
Polyprotic acids of the type H2M constitute two ionizable protons. As a result, on titration with a base, they exhibit two equivalence points in the titration curve. During titration, the species H2M, HM−, and M2− will be present in the solution at different points. The fractions of H2M, HM−, and M2− present at the various instances of the titration are denoted by α0, α1, and α2, respectively.
A graph with the alpha values is plotted against the volume of...
A graph with the alpha values is plotted against the volume of...
519
Detergent Purification of Membrane Proteins
5.2K
Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
5.2K


