番茄表观基因组的基因组范围广泛的遗传修改是由转激活的细菌CG甲基转移酶引起的
Bapatla Kesava Pavan Kumar1,2, Sébastien Beaubiat3, Chandra Bhan Yadav1,4
1Institute of Plant Sciences, Agricultural Research Organization, Volcani Center, Derech Hamacabim 68, Rishon Lezion, Israel.
将细菌CG特异性甲基转移酶引入番茄植物,稳定地改变了它们的全基因组DNA甲基化模式. 这创造了可遗传的表观遗传变异,为发现农业上有价值的番茄表观基因发现道路.
科学领域:
- 植物表观遗传学和基因组学
- 基因甲基化机制的DNA甲基化机制
- 可转移的元件沉声器可以转移.
背景情况:
- 植物的表观遗传变异是由DNA甲基化介导的.
- CG甲基化对于沉默可转移元素和调节基因表达至关重要.
- 甲基转移酶1在植物中维持CG甲基化.
研究的目的:
- 评估番茄基因组基因组稳定改变的可能性.
- 通过LhG4/pOP交换活化系统利用一种细菌CG特异性甲基转移酶 (M.SssI).
主要方法:
- 在番茄植物中表达细菌M.SssI甲基转移酶.
- 用M.SssI表达植物进行全基因组甲基组分析.
- 对因诱导高甲基化反应的基因表达变化的分析.
主要成果:
- 欧色区域和大多数基因成为CG高甲基化.
- 对于在转录起点附近易受CG超甲基化影响的基因,观察到基因表达变化.
- 异色区域显示轻微的CG低甲基化;一些M.SssI诱导的高甲基化是遗传的.
结论:
- 异质的M.SssI表达会诱导稳定,全基因组的表观遗传变异.
- 这种方法可以产生植物甲基化概况的遗传性变化.
- 创造表观遗传重组杂交系的潜力,以识别具有农业价值的表样样.
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