豆蛋白分离物的浸泡发酵及其对蛋白质功能,营养和挥发性概况的影响
Dai Shi1, Andrea K Stone1, Zahra Jafarian1
1Department of Food and Bioproduct Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Journal of food science
|May 20, 2024
概括
发酵的蛋白质分离与微生物,如阿斯伯吉勒斯或乳杆菌提高了蛋白质含量和可溶性. 然而,发酵减少了保持水分和消化能力,同时引入了有前途的新风味化合物.
科学领域:
- 食品科学与技术 食品科学与技术
- 微生物学 微生物学
- 蛋白质化学 蛋白质化学
背景情况:
- 脉冲发酵是一种清洁的技术,可以改善营养和功能质量.
- 与其他豆类相比,对豆蛋白分离物发酵的研究有限.
- 高蛋白质分离物 (>80%) 为微生物发酵提供独特的基质.
研究的目的:
- 为了研究潜水发酵使用特定微生物菌株对豆蛋白分离物的影响.
- 评估功能性质 (可溶性,水/油持久性,泡,乳液) 和消化性方面的变化.
- 识别挥发性化合物并评估改善感官属性的潜力.
主要方法:
- 在48小时内,使用黑色阿斯伯吉拉斯,亚斯伯吉拉斯奥里扎和植物乳杆菌 (Lactobacillus plantarum) 浸泡发酵豆蛋白分离物.
- 分析蛋白质含量,可溶性,油容量,水容量,泡容量和乳液稳定性.
- 评估蛋白质消化能力和识别挥发性化合物.
主要成果:
- 发酵增加了蛋白质含量,可溶性和油持有能力.
- 在受控发酵下保持泡能力,但泡/乳液稳定性和保持水分的能力下降.
- 蛋白质的消化能力降低,可能是由于和氨酸的增加;发现了具有潜在风味益处的新型挥发性化合物.
结论:
- 发酵的豆蛋白分离物表现出功能性质的改变,蛋白质含量和溶解度增加,但保持水分和消化能力降低.
- 特定的微生物菌株影响发酵结果,影响质量属性.
- 发酵的豆蛋白分离物显示出在食品应用中改善感官特征的潜力,这需要进一步研究微生物代谢.
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