一种经过验证的超高性能液体色谱与并联质谱法用于量化巴西绿色的主要化合物
Mário Ferreira Conceição Santos1, Giulia Stavrakas Mirada1, Joice Olinda do Couto2
1Center of Exact, Natural and Health Sciences, Federal University of Espírito Santo - UFES, Alto Universitário, Alegre, Espírito Santo, Brazil.
Natural product research
|May 20, 2024
概括
一种新的UPLC-MS/MS方法精确地分析了巴西绿色,一种具有药用用途的天然产品. 这种经过验证的技术可确保对质量控制的关键化合物的可靠量化.
科学领域:
- 分析化学 分析化学
- 药理学是指药理学,它是指药理学.
- 自然产品分析 自然产品分析
背景情况:
- 巴西绿色以其多样化的生物活动和药理性质而闻名.
- 植物化学研究将这些特性与特定化合物联系起来,需要精确的分析方法.
- 的醇提取物需要强大的分析技术来评估质量.
研究的目的:
- 开发和验证巴西绿色提取物的精确分析方法.
- 为定量分析建立可靠的UPLC-MS/MS (超高性能液体染色学-并联质谱) 方法.
- 确定方法稳定性的关键参数,并评估其灵敏度和准确性.
主要方法:
- 开发和验证一种超高性能液体染色学-并联质谱法 (UPLC-MS/MS) 方法.
- 使用维拉特化物作为质量和定量分析的内部标准.
- 评估方法的精度,稳定性,检测/量化极限和回收率.
主要成果:
- 开发的UPLC-MS/MS方法表现出高精度,相对标准偏差 (RSD) <4%的日内和日间分析.
- 波长检测和流量被确定为影响方法稳定性的关键参数.
- 该方法实现了低检测极限 (0.30039.500 ng/mL) 和定量 (1.40085.00 ng/mL),具有优异的恢复率 (94119%).
结论:
- 经过验证的UPLC-MS/MS方法对于分析巴西绿色是精确,敏感和可靠的.
- 该方法为提取物质的质量控制和植物化学表征提供了一个强大的工具.
- 这些发现支持准确评估巴西绿色中生物活性化合物的药用用途.
相关概念视频
01:37NMR Spectroscopy of Benzene Derivatives
Simple unsubstituted benzene has six aromatic protons, all chemically equivalent. Therefore, benzene exhibits only a singlet peak at δ 7.3 ppm in the 1H NMR spectrum. The observed shift is far downfield because the aromatic ring current strongly deshields the protons. Any substitution on the benzene ring makes the aromatic protons nonequivalent, and the protons split each other. The peak is, therefore, no longer a singlet and the splitting pattern and their associated coupling constants depend...
01:14Gas Chromatography–Mass Spectrometry (GC–MS)
Gas chromatography–mass spectrometry (GC–MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall. The coating...
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall. The coating...
01:12Chromatographic Methods: Classification
Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...
Chromatographic techniques are typically named by...
01:13Gas Chromatography: Introduction
Gas chromatography (GC) is a technique for separating and analyzing volatile compounds in a sample. Its primary purpose is to identify and quantify components in complex mixtures, making it essential in fields such as environmental analysis, pharmaceuticals, and petrochemicals. GC is also called vapor-phase chromatography (VPC) or gas-liquid partition chromatography (GLPC).
In GC, a sample is vaporized and mixed with an inert carrier gas (the mobile phase), which transports it through a column.
In GC, a sample is vaporized and mixed with an inert carrier gas (the mobile phase), which transports it through a column.
01:15High-Performance Liquid Chromatography: Types of Detectors
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...
01:18Supercritical Fluid Chromatography
Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...
SFC utilizes a supercritical fluid mobile phase,...