卡德林表达是由周周血管矩阵中纤维细胞的机械表现型调节的
Vaishali Bala1, Vidhi Patel1, Mary Kathryn Sewell-Loftin1,2
1Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, Alabama, USA.
Cells, tissues, organs
|May 20, 2024
概括
机械活跃的 stromal 细胞通过改变卡德林表达来影响血管生长. 这项研究揭示了流体细胞机械特性如何影响3D模型中的血管化,为组织工程提供了洞察力.
科学领域:
- 生物医学工程 生物医学工程
- 细胞生物学 细胞生物学
- 组织工程是组织工程.
背景情况:
- 流体细胞的机械力量调节血管生长.
- 卡德林是机械传感器,对血管完整性至关重要.
- 流体细胞机械在血管干素信号传递中的作用在很大程度上是未知的.
研究的目的:
- 调查树皮细胞机械表型与素表达之间的关系.
- 在3D微组织模型中描述树皮细胞的机械特性.
- 了解流体细胞机制如何影响血管化.
主要方法:
- 利用珠子位移试验来量化由树皮细胞引起的矩阵扭曲.
- 采用了3D微组织模型,包括人类心室内膜 (NHCF),皮肤 (NHDF),肺 (NHLF),癌症相关 (CAF) 和正常乳腺纤维细胞 (NBF).
- 同培养的内皮细胞 (EC) 与机械活跃或不活跃的树皮细胞,通过免疫光测量N-Cadherin (N-Cad),OB-Cadherin (OB-Cad) 和血管内皮卡德林 (VE-Cad) 的表达.
主要成果:
- 根据矩阵移位识别出机械活跃的树皮细胞 (CAF,NHCF,NHDF) 和机械不活跃的细胞 (NHLF,NBF).
- 与单独EC相比,在EC与CAF或NBF的共同培养中观察到减少VE-Cad表达.
- 在N-Cad和VE-Cad之间显示出强烈的相关性,表明局部化,并将增加的矩阵变形与增强的血管生长联系起来.
结论:
- 干细胞机械表型,以及分泌的因素,与卡德林调节和血管化潜力有关.
- 这些发现为控制组织工程支架中的血管生长提供了新的见解.
- 这项研究揭示了在发育血管床的紧密结节调节的潜在机制.
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