PagMYB180通过树的ROS/PCD依赖途径来调节偶然根植
Botong Tong1, Yingli Liu2, Yucheng Wang3
1State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University and Chinese Academy of Forestry, Harbin 150040, China.
概括
研究人员确定PagMYB180是树中偶然根 (AR) 形成的关键调节者. 这种转录因子通过活性氧物种 (ROS) 和编程细胞死亡 (PCD) 途径影响根部发育.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 林业林业 林业 林业 林业
背景情况:
- 偶然的根 (AR) 形成对于木质植物的植物性繁殖至关重要.
- 反应性氧物种 (ROS) 在植物根的发育中充当信号分子.
研究的目的:
- 确定和描述R2R3-MYB转录因子PagMYB180在混合 (Populus alba × Populus glandulosa) 中调节AR形成中的作用.
主要方法:
- 基因表达分析 (转录组,RT-qPCR) 来研究PagMYB180.0.
- 通过主导抑制和过度表达PagMYB180.0.的功能分析.
- 评估AR和侧根 (LR) 发育,ROS水平和编程细胞死亡 (PCD).
主要成果:
- PagMYB180作为一个转录抑制剂,局部存在于核中.
- PagMYB180抑制/过度表达减少了AR数量,但增加了AR和LR长度.
- PagMYB180影响根皮层细胞中的ROS水平和PCD,影响AR发育.
结论:
- PagMYB180是一种新型调节器,用于调节树中AR的形成.
- 这项研究表明,PagMYB180通过ROS/PCD依赖的途径调解AR的发展.
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