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相关概念视频

Cell Signaling in Plants01:25

Cell Signaling in Plants

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Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
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Riboswitches01:56

Riboswitches

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Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
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Reporter Genes02:11

Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Transgenic Plants02:50

Transgenic Plants

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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
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相关实验视频

Updated: Jun 25, 2025

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
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基于CRISPRi的电路用于控制植物中的基因表达.

Muhammad Adil Khan1,2, Gabrielle Herring1,2, Jia Yuan Zhu1,2

  • 1Australian Research Council Centre of Excellence in Plant Energy Biology, School of Molecular Sciences, The University of Western Australia, Perth, Western Australia, Australia.

Nature biotechnology
|May 20, 2024
PubMed
概括

研究人员为植物开发了一种CRISPR干扰 (CRISPRi) 基因电路平台,可以精确控制基因表达. 这种模块化系统为各种植物物种的合成生物学应用提供了可编程性和可逆性.

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科学领域:

  • 合成生物学 合成生物学
  • 植物生物技术 植物生物技术
  • 分子生物学分子生物学

背景情况:

  • 植物中合成基因电路的构建受到适应性和独立组件稀缺的阻碍.
  • 现有的方法缺乏复杂的遗传编程所需的正交和模块化.

研究的目的:

  • 在植物中建立基于CRISPR干扰 (CRISPRi) 的可逆基因电路平台.
  • 为复杂的基因表达控制设计一个可抑制的促进器和逻辑门的工具包.

主要方法:

  • 在Arabidopsis thaliana原塑体中使用工程可抑制的促进体实现CRISPRi系统.
  • 开发 NOT 和 NOR 逻辑门,优化单导 RNA 表达式.
  • 在稳定转变的阿拉比多普西斯和跨物种原质体 (Physcomitrium patens,Triticum aestivum,Brassica napus) 中测试CRISPRi门的功能.

主要成果:

  • 在Arabidopsis原生体和整个植物中成功构建和演示NOR门,展示了可编程性和可逆性.
  • 在多种植物物种中验证CRISPRi逻辑门活动.
  • 通过集成多个NOR门来创建OR,NIMPLY和AND逻辑函数,确认系统模块化.

结论:

  • 开发的CRISPRi平台为植物提供直角,紧,可逆,可编程和模块化合成基因电路.
  • 这种系统能够对基因表达进行复杂的时空控制,进步了植物合成生物学.