前后Wnt信号网络保护间接发育的海刺和半弦状胚胎之间的间接发育
Jennifer L Fenner1, Callum Newberry1, Callie Todd1
1Department of Biological Sciences, Auburn University, Auburn, AL 36849, USA.
Integrative and comparative biology
|May 20, 2024
概括
海的前后轴形成涉及Wnt信号通路. 这项研究发现了类似的Wnt基因表达模式在半弦动物中,这表明了动物身体计划进化的保存机制.
科学领域:
- 进化发育生物学 进化发育生物学
- 进行比较的基因组学.
- 动物的身体计划进化 动物的身体计划进化
背景情况:
- 建立胚胎轴对于动物体型的形成至关重要.
- 在海中,Wnt信号通路 (Wnt/β-catenin,Wnt/JNK,Wnt/PKC) 控制了前后轴形成.
- 了解这些机制在deuterostomes的保护是跟踪AP轴进化的关键.
研究的目的:
- 为了研究海刺前后 (AP) Wnt 模式基因的保全,在半弦状动物中进行基因正统学.
- 为了比较海和半弦动物之间的Wnt通路组件的时空基因表达.
- 通过研究Ambulacrarians来告知弦状动物体图的进化.
主要方法:
- 测试了海AP Wnt的空间时间基因表达,在半弦状物中对基因正统的模式进行测试 Schizocardium californicum.
- 专注于早期的AP轴形成和前部神经切皮 (ANE) 模式.
- 对比了Wnt8,Frizzled5/8,Wnt1,Wnt2和Wnt4的表达模式.
主要成果:
- 在早期AP形成期间,前部神经皮质的定位与间接发育的半弦状动物和海相似.
- 在海中,Wnt8和Frizzled5/8的表达是关键的ANE模式驱动因素,在半弦胚胎中被保存.
- 在早期Wnt基因 (Wnt1,Wnt2,Wnt4) 的胚胎表达中观察到分歧.
结论:
- 对于AP轴图案的Wnt信号网络显示,与海相比,在间接发育的半弦状动物中存在显著的保护.
- 这种保护为未来对半弦状动物的功能研究提供了基础.
- 这些发现有助于了解体前后轴在体前后轴的演变.
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