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单细胞转录组揭示了在人类结脊髓炎中失调的RNA结合蛋白表达模式和功能

Zheng Ren¹, Chenyang Li², Jing Wang¹

¹Xinjiang Institute of Spinal Surgery, Sixth Affiliated Hospital of Xinjiang Medical University, Ürümqi, Xinjiang, China.

Frontiers in medicine

|May 21, 2024

概括

RNA结合蛋白 (RBPs) 在化脊髓炎 (AS) 的免疫细胞中显示出不同的表达模式. 它们的失调与AS发展有关,突出显示RBP是这种炎症性疾病的潜在治疗点.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

化脊柱炎 (AS) 是一种慢性炎症性疾病,主要影响轴骨架.
免疫细胞失调在AS的发病过程中起着至关重要的作用.
RNA结合蛋白 (RBPs) 是基因表达和细胞功能的关键调节者,但它们在AS免疫中的特定作用尚未完全理解.

研究的目的:

研究AS患者在各种免疫细胞类型中RBPs的表达特征和调节模式.
确定关键的RBP参与AS的发病和进展.
阐明RBPs在AS相关免疫反应中的潜在功能作用.

主要方法:

使用单细胞RNA测序 (scRNA-seq) 和大量RNA测序 (RNA-seq) 来自AS患者和健康对照组 (HC) 的数据.
进行了相关性分析,以确定差异表达的RBP及其相关的免疫细胞群.
分析了RBPs在关键信号通路中对AS相关的潜在参与.

主要成果:

与HC相比,AS患者观察到免疫细胞组成的显著变化,包括B细胞,树突细胞和CD14+单细胞的增加,以及血小板和NK细胞的减少.
特定的RBP,如B细胞中的DDX3X和SFPQ,以及CD8+T细胞中的EIF2S3和SRSF7,被确定为在AS中对免疫细胞分化和功能的潜在重要.
差异表达的可变拼接基因 (RASG) 参与了AS的转录调节,蛋白质酸化和血管生成等途径.

结论:

在AS中,RBP在不同类型的免疫细胞中表现出特定的表达特征和调节功能.
RBPs的异常表达和失调与AS的发生和发展密切相关.
准RBPs可能为管理AS提供一种新的治疗策略.

关键词:

化脊柱炎 (AS) 是一种疾病.B 细胞 B 细胞 B 细胞 CD8+ T 细胞 CD8+ T 细胞基因基因基因基因基因它们是RNA结合蛋白 (RNA-binding proteins,简称RBPs).单细胞RNA测序 (scRNA-seq) 是一种

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