来自适应盐水的灌木的真菌内植物诱导番茄幼苗的盐水应激耐受性
Priscillar Mumo Mutungi1,2, Vitalis Wafula Wekesa3, Justus Onguso1
1Jomo Kenyatta University of Agriculture and Technology, Institute for Biotechnology Research, P.O. Box 62000-00200, Nairobi, Kenya.
FEMS microbes
|May 21, 2024
概括
来自耐盐植物的内菌可以帮助番茄等作物抵御土壤盐度. 四种特定的真菌在盐压力下增强了番茄种子的发芽和幼苗的生长,为农业提供了可持续的解决方案.
科学领域:
- 农业科学 农业科学
- 植物病理学 植物病理学
- 微生物学 微生物学
背景情况:
- 全球粮食需求需要到2050年增加产量,但气候变化,特别是土壤盐化,威胁着作物产量.
- 居住在适应盐的植物中的内菌是赋予敏感作物盐度耐受性的潜在候选者.
研究的目的:
- 从马加迪湖的耐盐灌木中分离内菌并评估它们在番茄中诱导盐度应激耐受性的潜力.
- 评估在盐水条件下增强番茄植物弹性方面的选定真菌内菌的有效性.
主要方法:
- 从适应盐的宿主植物中分离和描述内菌.
- 隔离物的选在高盐度 (1.5M NaCl) 下生长和酶生产 (氨酶,蛋白酶,细胞酶,酸盐溶解).
- 在温室中对四种选定的内植物 (Cephalotrichum cylindricum,Fusarium equiseti,Fusarium falciforme,Aspergillus puniceus) 进行评估,以确定它们殖民番茄幼苗的能力,并赋予耐盐度.
主要成果:
- 获取了60种内性真菌分离物,主要来自Ascomycetes (95%),其中大多数来自根 (48.3%).
- 只有8个分离物耐受1.5M NaCl;四个被选择进行进一步测试.
- 用四种选择的内植物进行注射,可使番茄种子发芽率提高23%,并提高了苗木的叶绿素和生物质含量,同时在盐度压力下降了过氧化水平.
结论:
- 四种真菌内植物 (Cephalotrichum cylindricum,Fusarium equiseti,Fusarium falciforme和Aspergillus puniceus) 成功地殖民番茄植物,并赋予了它们显著的盐分耐受性.
- 这些内植物显示出作为生物注射剂的潜力,以减轻盐度压力,在盐受影响的农业土壤中种植番茄的盐度压力.
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