在宿主中编辑RNA lncRNAs作为SARS-CoV-2变体的潜在调节器-宿主免疫反应动态
Partha Chattopadhyay1,2, Priyanka Mehta1,2, Kanika1
1Division of Immunology and Infectious Disease Biology, INtegrative GENomics of HOst-PathogEn (INGEN-HOPE) laboratory, CSIR-Institute of Genomics and Integrative Biology (CSIR-IGIB), Mall Road, Delhi 110007, India.
iScience
|May 21, 2024
概括
在长非编码RNA (lncRNAs) 中的宿主RNA编辑在COVID-19变种中有所不同. 德尔塔变种患者的RNA编辑率较低,影响了与感染相关的途径和疾病严重程度.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 病毒学 病毒学
背景情况:
- 宿主和病毒RNA编辑在感染反应中至关重要,但宿主RNA编辑机制尚未完全理解.
- 长非编码RNAs (lncRNAs) 参与细胞过程,但它们在病毒感染期间编辑RNA中的作用需要进一步调查.
研究的目的:
- 为了研究不同COVID-19变种 (PreVOC,Delta,Omicron) 中 lncRNAs中的差异性RNA编辑模式.
- 探索lncRNA编辑,宿主基因表达和COVID-19疾病严重程度之间的关系.
主要方法:
- 来自211名住院COVID-19患者的样本的RNA测序 (RNA-seq) 分析.
- 在感染不同SARS-CoV-2变种的患者群体中对lncRNAs中的RNA编辑频率和模式进行比较分析.
- 生物信息分析以确定差异表达的基因和途径.
主要成果:
- 在COVID-19变种中观察到lncRNA编辑频率和模式的显著差异.
- 与PreVOC和Omicron组相比,Delta变异组在lncRNA中显示较低的RNA编辑.
- 特定的lncRNAs (UGDH-AS1,NEAT1) 显示了高编辑频率;ADAR1,APOBEC3A/G和重复丰度是潜在的调节器.
- 在德尔塔变种组中,发现了与感染相关的通路的下调.
结论:
- 宿主lncRNA编辑影响RNA稳定性,microRNA结合和基因表达,从而调节COVID-19疾病的严重程度.
- 不同的lncRNA编辑模式可以作为COVID-19变种影响和疾病进展的生物标志物.
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