在抗原受体位置的多个谱系特定的表观遗传景观
Xiang Qiu1,2, Guanxiang Liang1, Weiqiang Zhou3
1Department of Biomedical Sciences, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
概括
这项研究揭示了胚胎干细胞 (ES) 中的抗原受体 (AgR) 位点表现出类似于淋巴细胞的活跃表观遗传标记,这表明在多能性和组织特异激活中发挥了作用.
科学领域:
- 免疫学 免疫学 免疫学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 发育生物学 发展生物学
背景情况:
- 在B细胞和T细胞上的抗原受体 (AgRs) 对适应性免疫至关重要.
- 在淋巴细胞中,B细胞受体 (BCR) 和T细胞受体 (TCR) 基因的表观遗传调节已知,但在其他细胞类型中却不那么知名.
- 了解不同细胞类型中AgR位点的表观遗传特征对于理解免疫系统发育和细胞分化至关重要.
研究的目的:
- 在淋巴细胞,胚胎干细胞 (ES细胞) 和神经元中研究和比较BCR和TCR位点的基因组修饰和转录标志物.
- 在不同细胞类型中识别不同的和重叠的表观遗传模式.
- 探索ES细胞中的表观遗传特征与淋巴细胞激活之间的潜在关系.
主要方法:
- 在BCR和TCR位点对基质子修饰和转录标记物的分析.
- 通过亲B细胞,DPT细胞,成熟的CD4+T细胞,ES细胞和神经元进行比较.
- 检查转录因子 (Oct4,Sox2,Nanog,YY1) 和染色体重塑剂 (Brg1,CTCF) 的结合模式.
主要成果:
- BCR和TCR位点在淋巴细胞中显示出活跃的表观遗传标记,与活跃的基因表达一致.
- 神经元中的AgR位点在很大程度上在表观遗传上是不活跃的.
- 令人惊的是,ES细胞在AgR位点表现出广泛的活性表观遗传标记,与亲B细胞相比,具有明显的模式.
- 特定的转录因子和染色体重塑剂结合模式在ES和亲B细胞之间存在差异,而CTCF结合显示重叠.
结论:
- 在ES细胞中的AgR位点上的表观遗传景观可能有助于它们的多能状态.
- 淋巴细胞中明显的表观遗传特征促进了AgR位点的组织特异激活.
- 这项研究突出了非淋巴细胞,特别是ES细胞中AgR位点的新型表观遗传调节.
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