相关实验视频
Updated: Jun 25, 2025

13:00
Engineering Antiviral Agents via Surface Plasmon Resonance
Published on: June 14, 2022
2.3K
诺维林-N与N-乙-d-葡萄糖胺结合需要在两个不同的原体上建立碳水化合物结合位点
Irene Maier1,2, Georg Kontaxis3, Christian Zimmermann4
1Department of Environmental Health Sciences, Fielding School of Public Health, University of California, Los Angeles, 650 Charles E. Young Dr. South, Los Angeles, California 90095, United States.
Biochemistry
|May 21, 2024
概括
诺维林-N (CV-N) 变体对病毒葡萄糖蛋白和人类T细胞的结合发生了变化. 工程二次体对曼诺和N-乙葡萄糖胺具有特定的亲和力,影响抗病毒和淋巴细胞激活活动.
科学领域:
- 生物化学 生物化学
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
背景情况:
- 诺维林-N (CV-N) 是一种强有力的抗病毒药物,它与包裹病毒上的高曼诺糖寡糖化合物结合.
- CV-N 具有两个碳水化合物结合位点,对曼诺斯部分有不同的亲和力.
- 了解CV-N的结合特性对于开发新型抗病毒疗法至关重要.
研究的目的:
- 研究CV-N变体和二元体对特定碳水化合物的结合特性.
- 评估工程CV-N构造对HIV-1的抗病毒活性.
- 探索CV-N通过碳水化合物交叉链接来激活淋巴细胞的潜力.
主要方法:
- 用局部导向的突变发生法来创建具有改变氨基酸残留的CV-N变体.
- 构建和表达了CV-N (CVN2) 和域互换二极体的并列重复.
- 使用诸如表面等离子体共振 (SPR) 等技术来确定碳水化合物结合亲和力.
- 进行了针对HIV-1的抗病毒活性和淋巴细胞激活测试.
主要成果:
- CV-N变种显示出与N-乙曼诺胺 (ManNAc) 和N-乙-d-葡萄糖胺 (GlcNAc) 的特定结合.
- CVN2表现出低亲和力结合于二糖化和GlcNAc的结合.
- 设计的CV-N二元体显示出高亲和度的 GlcNAc 结合,并有可能在人类 T 细胞上交叉链接碳水化合物.
结论:
- 工程 CV-N 变体和二极管提供可调节的碳水化合物结合特异性.
- 这些修改后的CV-N分子对向的抗病毒疗法和调节免疫反应充满希望.
- 该研究强调了CV-N在模仿治疗应用中的生物相互作用方面的潜力.
相关概念视频
Glycocalyx and its Functions
4.0K
The glycocalyx is a carbohydrate-rich, fuzzy-appearing layer on the outer surface of the cell membrane. It is highly hydrophilic, because of this it attracts large amounts of water to the cell's surface. This aids the cell's interaction with the watery environment and also helps it to obtain substances dissolved in the water. It is also important for cell identification, self/non-self determination, and embryonic development and is used in cell-to-cell attachments to form tissues.
4.0K
Oligosaccharide Assembly
2.8K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Multiple sugar molecules that may or may...
2.8K
Membrane Carbohydrates
5.4K
The plasma membrane is a dynamic barrier composed of lipids, proteins, and carbohydrates. It is the epicenter of many cellular processes required for cell growth and survival. Carbohydrates have unique structural and chemical properties that help the plasma membrane to carry out its functions effectively.
Membrane carbohydrates do not have any hydrophobic region and are exclusively located on the cell's outer surface. The addition of sugar molecules or glycosylation of proteins happens in...
Membrane carbohydrates do not have any hydrophobic region and are exclusively located on the cell's outer surface. The addition of sugar molecules or glycosylation of proteins happens in...
5.4K
Protein Glycosylation
6.9K
Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
Glycosylation occurs in...
6.9K
Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
Protein Complexes with Interchangeable Parts
2.5K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.5K

