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识别了Shewanella菌LambdaSo溶解系统的组件

Svenja Thöneböhn¹, Dorian Fischer¹, Vanessa Kreiling¹

¹Institute of Microbiology and Molecular Biology, Justus-Liebig-Universität Gießen, Gießen, Germany.

Journal of bacteriology

|May 21, 2024

概括

菌体LambdaSo使用了pinholin/信号释 (SAR) 解酶系统和两组分的spanin用于阴性细菌溶解. 一种新型的菌体蛋白,Lcc6,也对内溶素释放至关重要,扩大已知的溶解因子.

科学领域:

微生物学微生物学
分子生物学分子生物学
病毒学病毒学

背景情况:

菌体诱导的格拉姆阴性细菌溶解通常涉及荷林,内林和斯班林,以破坏细胞外.
众所周知,Shewanella oneidensis*中的LambdaSo传染体具有潜在的holin和endolysin基因,但缺乏明显的素系统.

研究的目的:

为了研究Shewanella oneidensis* prophage LambdaSo的溶解机制.
识别所有涉及LambdaSo介导细胞溶解和菌体退出的必需的菌体编码蛋白质.

主要方法:

基因组注释和LambdaSo.So的分析.
菌素突变的构造和表征.
显微镜观察细胞溶解.
生物化学试验用于研究蛋白质功能.

主要成果:

LambdaSo采用了pinholin/信号释放 (SAR) 的内溶酶系统,用于细胞壁降解和质子泄漏.
在嵌套的开放式读取中,确定了一种两组分的素系统 (Rz/Rz1).
发现以前未被描述的菌体蛋白Lcc6对于溶解至关重要,它在细胞质膜上起作用,促进内溶素的释放.
兰巴索介导的溶解至少需要四种蛋白质因子:pinholin,SAR内素,spanin和Lcc6.

结论:

LambdaSo 的溶解机制比最初预测的要复杂得多,除了正规的溶解成分外,还需要一种新的蛋白质因子 (Lcc6).
这些发现扩大了已知的菌体溶解蛋白的范围,并为复杂的菌体-宿主细胞外破坏过程提供了洞察力.

关键词:

这就是SAR-endonuclease.霍林·霍林 (Holin Holin) 是一个著名的艺术家.预言预言预言预言在西班牙,西班牙人.

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