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在Cordyceps militaris中通过定数感应系统增强了多糖类的产生

Huang Qiao¹, Jianshu Chen¹, Shengli Yang¹

¹The College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, China.

Journal of basic microbiology

|May 21, 2024

概括

研究人员设计了Cordyceps militaris,以使用定数感应 (QS) 系统来提高细胞外多糖 (EPS) 产量. 这种基因改造显著增加了EPS产量,为大规模的真菌多糖化物生产提供了一种新方法.

科学领域:

生物技术是生物技术.
菌类学菌类学是指菌类学.
合成生物学合成生物学

背景情况:

来自Cordyceps militaris的细胞外多糖 (EPS) 具有宝贵的生物活动.
优化EPS生产对于工业应用至关重要.
了解真菌的定数感应 (QS) 机制可以解锁新的生产策略.

研究的目的:

为了增强Cordyceps militaris.中的细胞外多糖 (EPS) 生产.
设计一个定数感应 (QS) 系统来调节关键生物合成酶基因.
调查芳香氨基酸对EPS生产的影响.

主要方法:

构建了一个QS系统来控制EPS生物合成基因的表达 (糖突变酶,糖突变酶,糖突变酶,糖突变酶,多糖合成酶,UDP-葡萄糖4-表皮酶).
产生并选了七种重组的C. militaris菌株,以增加EPS产量.
评估了托芬补充剂对工程CM-pgm-2菌株EPS产量的影响.

主要成果:

七个重组菌株的EPS度高于野生型C. militaris. 的EPS度.
CM-pgm-2菌株获得了最高的EPS产量 (3.82g/L),是野生类型的1.52倍.
托芬 (45 mg/L) 进一步增加了CM-pgm-2中的EPS产量,达到4.75 g/L (1.90倍增长与野生类型相比).

结论:

通过QS系统对关键酶基因的过度表达有效地提高了C. militaris.中的EPS产量.
芳香氨基酸,如托,可以通过工程QS系统进一步调节EPS产量.
这项研究为大规模EPS生产提供了一种可行的方法,并推进了真菌QS研究.

关键词:

农业细菌的转化转化科尔迪塞普斯军用菌 (Cordyceps militaris) 是一种植物.动态控制的动态控制细胞外的多糖化物.执法制感应系统是指执法制感应.

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