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在SEPALLATA驱动的MADS转录因子四重化是内旋花器官发育所需的

Veronique Hugouvieux¹, Romain Blanc-Mathieu¹, Aline Janeau¹

¹Laboratoire de Physiologie Cellulaire et Végétale, Université Grenoble-Alpes, CNRS, CEA, INRAE, IRIG-DBSCI, 17 rue des Martyrs, 38000 Grenoble, France.

|May 21, 2024

概括

SEPALLATA (SEP) MADS转录因子的四重化对于Arabidopsis.com的花器官身份至关重要. 虽然SEP二极体结合了DNA,但四极体化对于正确的花朵发育和花干系的确定性至关重要.

科学领域:

植物生物学植物生物学
分子遗传学分子遗传学
发育生物学是发展生物学.

背景情况:

MADS转录因子,特别是SEPALLATA (SEP) 子家族,是植物繁殖和花朵发育的重要调节者.
在MADS复合体中,SEP蛋白质充当关键组织者,能够形成异构体和异构四聚体.
之前的研究表明SEP蛋白在花器官发育和花系统确定性中的作用,但二维DNA结合对花器官身份的充分性仍然不清楚.

研究的目的:

为了研究SEPALLATA (SEP) 蛋白质四重化在花器官身份和花髓系确定性中的功能必要性.
为了确定含有SEP的异构体是否足以启动花器官识别程序.
阐明SEP四重化在花朵发育过程中全基因组DNA结合和基因调节中的作用.

主要方法:

用全基因组DNA结合研究 (ChIP-seq) 来绘制SEP3结合位点的地图.
对SEP3/AGAMOUS (AG) 四聚化域的高分辨率结构分析提供了结构性见解.
基于结构的突变发生在SEP3上进行,以创建四重化受损突变.
在阿拉比多普西斯 (Arabidopsis thaliana) 突变种 (sep1 sep2 sep3 和 sep1 sep2 sep3 ag-4) 的补充实验中,使用转变的SEP3突变种进行了实验.

主要成果:

像野生型SEP3一样,SEP3异构体在体外和体内都表现出结合DNA的能力.
SEP3二元识别了野生类型SEP3占据的基因组范围绑定位的很大一部分.
发现SEP3的四重化对于花干系的确定性至关重要.
最重要的是,四重化还被证明是花的第二,第三和第四个旋转中建立花器官身份的必不可少.

结论:

SEP蛋白质四重化是建立Arabidopsis花器官身份的关键决定因素,超出了它在花介质决定中的作用.
虽然SEP异构体可以结合DNA并识别调节元素,但需要更高阶的复合体形成 (四极化) 来完全激活植物器官身份基因的转录.
这些发现澄清了植物繁殖发育中的MADS复杂功能背后的分子机制.

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