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控制精子功能的关键调节蛋白QRICH2具有强烈的抗氧化特性,增强了精子的活力

Guohui Zhang¹, Dongsheng Xiong², Fei Ye²

¹Sichuan Provincial Key Laboratory for Human Disease Gene Study and the Center for Medical Genetics, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, University of Electronic Science and Technology, Chengdu 610072, China; Key Laboratory of Reproductive Medicine, Center of Reproductive Medicine, Sichuan Provincial Maternity and Child Health Care Hospital, Chengdu 610045, China.

Reproductive biology

|May 21, 2024

概括

富含谷氨胺的蛋白2 (QRICH2) 保护精子免受氧化损伤. 缺乏QRICH2会增加活性氧物种 (ROS),导致DNA损伤,亡和精子数量减少,但其N端蛋白可能有助于精子的保存.

科学领域:

生殖生物学生殖生物学
分子遗传学分子遗传学
氧化应激研究研究氧化应激研究

背景情况:

男性不孕症影响15%的夫妇,遗传因素起着重要作用.
富含谷氨酸蛋白2 (QRICH2) 基因中的功能丧失变异与男性不孕症和精子鞭毛异常有关.
小鼠的QRICH2缺乏导致精子数量减少,但潜在的机制需要阐明.

研究的目的:

调查QRICH2影响男性生育能力的机制.
确定QRICH2在保护精子发生过程中的氧化应激中的作用.
评估QRICH2蛋白在精子保存中的潜在治疗应用.

主要方法:

在Qrich2淘汰赛小鼠的丸上进行了血氧氨 (HE) 染色和免疫光染色.
流细胞计和Qrich2淘汰赛小鼠精子的单个精子代谢分析.
在试验室中用纯化的QRICH2 N端蛋白对精子进行化.

主要成果:

在体内和体外,QRICH2具有显著的抗氧化能力.
Qrich2淘汰赛小鼠表现出高反应性氧物种 (ROS) 水平,精子体的DNA损伤,自和亡的增加,以及精子数量的减少.
QRICH2 N端蛋白在体外显示出强大的抗氧化活性,增强了精子的活力和运动性.

结论:

QRICH2对于通过增强抗氧化能力来保护精子体免受ROS诱导的损伤至关重要,从而确保精子的生存和运动.
QRICH2 缺乏导致男性不孕症通过氧化应激,DNA 损伤和亡.
N端的QRICH2蛋白显示出作为保护性添加剂的潜力,用于精子保存和冷保存.

关键词:

这是一个QRICH2符号.ROS,抗氧化剂,精子的运动性,细胞亡.

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