低频磁场有助于阿斯伯吉路斯尼格尔 (Aspergillus niger) 产生酸性蛋白酶
Jinlong Liu1,2, Dongxu Wang3, Hong Wang3
1College of Food and Biology, Hebei University of Science and Technology, ShiJiaZhuang, 050018, China. jlliu18@126.com.
Archives of microbiology
|May 21, 2024
概括
低频磁场显著提高了Aspergillus niger中的酸蛋白酶的产生. 交替磁场显示出更高的效率,增强了工业应用的酶活性和生物质.
科学领域:
- 生物技术是生物技术.
- 酵素工程是什么意思 酵素工程
- 工业微生物学 工业微生物学
背景情况:
- 酸蛋白酶是食品加工和料添加剂中的关键酶.
- 微生物的酶生产对于工业应用至关重要.
- 优化发酵过程可以提高酶产量和效率.
研究的目的:
- 研究低频磁场 (LF-MF) 对Aspergillus niger的酸蛋白酶产生的影响.
- 评估交替磁场 (AMF) 和静态磁场 (SMF) 对酶活性和菌根生物质的影响.
- 阐明LF-MF辅助发酵的基础分子机制.
主要方法:
- 在不同的低频磁场条件下 (AMF和SMF) 种植Aspergillus niger.
- 测量菌根生物质和酸蛋白酶活性.
- 转录组分析以确定基因表达变化.
- 使用磁场处理原始酶溶液.
主要成果:
- 交替磁场 (AMF) 在低强度下比静态磁场 (SMF) 更有效.
- 与对照组相比,最佳的LF-MF条件导致酸蛋白酶活性增加91.44%,生物质增加16.31%.
- 原始酶溶液的磁场处理显示,酶活性最大增加了19.87%.
- 转录组学揭示了在LF-AMF治疗下与水溶酶和细胞生长相关的基因的显著上调.
结论:
- 低频磁场,特别是低强度的AMF,可以有效地增强Aspergillus niger的酸蛋白酶产生.
- LF-MF 影响 A. niger 的代谢途径,促进酶生产和细胞生长.
- 这项研究为磁场辅助发酵策略提供了宝贵的参考,以改善微生物酶生产.
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