蛋白质表达模式和金属代谢物在一个原生性雌雄性鱼,米田 (Monopterus albus) 的金属代谢物
Zhi He1,2, Feng Xiao1, Deying Yang1,2
1College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
BMC genomics
|May 21, 2024
概括
这项研究揭示了在田鱼性别变化期间的关键蛋白质变化,确定铜是关键的男性相关金属代谢物. 这些发现有助于我们更好地了解鱼类的生殖发育和荷尔蒙调节.
科学领域:
- 比较生理学比较生理学
- 生殖生物学 生殖生物学
- 蛋白质组学是指蛋白质组学.
背景情况:
- 田鱼 (Monopterus albus) 呈现出自然的雌雄性变异.
- 之前的研究已经确定了这种过渡的转录和蛋白质水平机制.
- 蛋白质水平的变化,特别是在性际阶段,仍未得到充分研究.
研究的目的:
- 综合分析田鱼在性别变化期间的蛋白质表达模式.
- 确定差异表达蛋白 (DEPs) 和它们在淋巴体发育中的潜在作用.
- 为了研究金属代谢物在性别逆转过程中的参与.
主要方法:
- 在五个性阶段 (OV,IE,IM,IL,TE) 进行性腺组织的非向蛋白质组测序.
- 差异性蛋白质表达分析以确定DEP.
- 用于验证候选蛋白质 (Igf2bp3,Tpi, (Cu-Zn) Sod1) 的西部抹杀方法.
- 对金属代谢物相关蛋白质的功能丰富分析.
- 金属代谢物 (Zn2+,Cu2+,Ca2+,Fe2+/Fe3+) 的量化.
主要成果:
- 在淋巴细胞组织中发现了5125种蛋白质和394种DEP.
- 在不同性阶段之间观察到显著的蛋白质表达变化.
- 与金属代谢物相关的蛋白质被丰富,特别是在性别变化的后期阶段.
- 铜 (Cu2+) 含量在性别变化期间显著上升,这表明铜在男性发育中的作用.
- 在两个与金属代谢物相关的基因/蛋白质中发现了蛋白质和mRNA之间的一致表达趋势.
结论:
- 在五个性阶段的田鱼淋巴发育过程中阐明了五个性阶段的蛋白质配置.
- 与金属代谢物相关的蛋白质和特定的金属离子 (Zn2+,Cu2+,Ca2+,Fe2+/Fe3+) 参与了性别变化过程.
- (Cu-Zn) Sod1和Mmp-9被确定为参与田鱼性别逆转的潜在关键蛋白质.
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