一个基于SAW的可编程控制RNA检测装置:在一个迷你室中快速在场细胞解-RNA捕获-RNA释放-PCR
Yupeng Yang1,2, Zenan Wang1, Hetao Xie2
1Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518000, P. R. China.
概括
一种新的表面声波 (SAW) 装置集成了病毒RNA检测步骤,减少了试剂的使用. 这种实验室在芯片上的技术为快速的治疗点传染病诊断提供了有前途的方法.
科学领域:
- 生物技术是生物技术.
- 纳米技术纳米技术
- 医学诊断 医学诊断 医学诊断
背景情况:
- 传统的病毒RNA检测方法是设备密集型的,需要熟练的人员.
- 需要快速,便携式和高效的诊断工具来治疗传染病.
研究的目的:
- 开发一种可编程,集成的基于表面声波 (SAW) 的设备,用于全面检测病毒RNA.
- 为了缩小和简化病毒RNA检测的过程,用于点的护理应用.
主要方法:
- 利用SAW诱导的液体旋转用于通过细胞微粒碰撞的细胞溶解.
- 采用SAW悬浮磁珠,用于高效的RNA捕获和化.
- 在单个微流体室内使用SAW产生的热进行集成PCR热循环.
主要成果:
- 该设备成功执行了所有病毒RNA检测步骤,包括溶解,捕获,化和PCR.
- 与手动提取 (2065μl) 相比,所需的试剂容量 (489μl) 显著减少.
- 取得的PCR结果与商业定量PCR (qPCR) 仪器可比.
结论:
- 基于SAW的实验室芯片设备展示了治疗点 (POC) 传染病测试的潜力.
- 这种新的方法可以快速有效地检测病毒RNA,克服传统方法的局限性.
相关概念视频
03:21RNA-seq
9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K
02:57Real Time RT-PCR
57.2K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
The real-time quantification of the number of amplified products is...
57.2K