在蔬菜/水果中使用液体染色学-双重质谱法与放电适配器接口来确定甲
Wing-Ming Chou1, Wen-Ling Lin1, Jenn-Feng Sheen1
1Department of Biotechnology, National Formosa University, Huwei, Taiwan.
Rapid communications in mass spectrometry : RCM
|May 22, 2024
概括
一种新方法可以准确检测产品中的甲 (CHT) 杀菌剂残留物. 这项技术使用液体染色学-并联质谱学 (LC-DA-MS/MS) 与经过修改的QuEChERS提取以实现更快,更便宜的分析.
科学领域:
- 分析化学 分析化学
- 环境科学 环境科学
背景情况:
- 甲 (CHT) 是一种用于控制植物疾病的广泛使用的杀菌剂.
- 对于食品中的CHT残留物及其代谢物4-基甲 (CHT-4-OH) 存在监管限制.
研究的目的:
- 开发和验证一种敏感的方法来确定蔬菜和水果中的CHT残留物.
- 提高CHT残留分析的效率并降低其成本.
主要方法:
- 使用修改后的快速,轻松,廉价,有效,坚固和安全 (QuEChERS) 方法,有效提取CHT和CHT-4-OH.
- 采用液体染色学-双重质谱法 (LC-MS/MS) 与一种新的修改后的放电适配器 (DA) 接口 (LC-DA-MS/MS) 进行分析.
- 通过同位素化,在3分钟内实现了CHT和CHT-4-OH的分离.
主要成果:
- 通过LC-DA-MS/MS方法证明了对CHT (2-3倍) 的改善离子信号.
- 在5-200 ng/g范围内达到5 ng/g的量化极限,具有很好的线性 (r2 > 0.99).
- 该方法表现出高精度和准确性 (在15%以内),成功检测了在野外种植的产品中高达2588.9 ng/g的CHT残留物.
结论:
- 开发的LC-DA-MS/MS方法与修改的QuEChERS相结合,有效量化蔬菜和水果中的CHT残留物.
- 该方法提供了由于简化的提取,同位素化和DA接口而降低了分析成本和时间.
- 满意的验证结果表明该方法对监管监测的可靠性.
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