ANSA-BSA协会的分析方面:一种热力学和合规的方法
Anu Jain1, Eva Judy1, Nand Kishore1
1Department of Chemistry, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
The journal of physical chemistry. B
|May 22, 2024
概括
这项研究介绍了ANSA染料作为与牛血清白蛋白 (BSA) 结合的ANS的优质替代品. ANSA简化了研究,并提高了检测血清白蛋白和部分折叠蛋白质的传感器准确性.
科学领域:
- 生物化学 生物化学
- 生物物理化学 生物物理化学
- 分子生物学分子生物学
背景情况:
- 牛血清白蛋白 (BSA) 与8-anilino-1-naphthalenesulfonic acid (ANS) 的相互作用已经得到充分证实.
- 由于ANS的溶解度较低,因此限制了其研究应用和用于检测血清白蛋白的传感器精度.
研究的目的:
- 为了研究ANSA染料和BSA之间的结合相互作用.
- 描述BSA在ANSA结合后的热力学和形状变化.
- 评估ANSA作为血清白蛋白和部分折叠蛋白质的潜在探针.
主要方法:
- 差分扫描热量计 (DSC) 用于评估蛋白质的热稳定性.
- 光光谱法以确定结合亲和力和pH依赖相互作用.
- 异热定位热度计 (ITC) 用于阐明结合模式.
- 时间解析单光子计数 (TCSPC) 用于动态研究.
- 分子对接以预测结合部位.
主要成果:
- 增加ANSA度显著提高了BSA的热稳定性.
- 随着pH的增加,ANSA与BSA的结合亲和力下降.
- ITC揭示了从单个结合点转向连续结合点的转变,pH值上升,表明了形状变化.
- 分子对接表明ANSA与BSA的疏水口袋结合,与离子中心相互作用.
结论:
- 由于其提高的溶解性和研究简单性,ANSA是对ANS进行研究BSA相互作用的可行和有利的替代方案.
- ANSA 结合会诱导 BSA 的形状变化,结合方式依赖于 pH.
- 作为检测血清白蛋白和识别部分折叠蛋白质构造的敏感探针,ANSA显示出有前途.
相关概念视频
Atomic Absorption Spectroscopy: Overview
2.0K
Atomic absorption spectroscopy (AAS) is a technique used to analyze elements by measuring electromagnetic radiation (EMR) absorbed by atoms, which causes them to transition to a higher-energy orbit. The most crucial step in AAS is atomization, where the analyte is converted into gas-phase atoms, typically through a flame or furnace. Some of these atoms become thermally excited in the flame, while most remain in the ground state.
When irradiated by EMR of a particular wavelength, these...
When irradiated by EMR of a particular wavelength, these...
2.0K
Atomic Emission Spectroscopy: Overview
2.1K
Atomic emission spectroscopy (AES) is an analytical technique used to determine the elemental composition of a sample by analyzing the light emitted from excited atoms. In AES, atoms in a sample are excited to higher energy levels by thermal energy from high-temperature sources, such as plasma, arcs, or sparks. When these excited atoms return to lower energy states, they emit light at specific wavelengths characteristic of each element. The resulting atomic emission spectrum, which consists of...
2.1K
Noncovalent Attractions in Biomolecules
50.3K
Noncovalent attractions are associations within and between molecules that influence the shape and structural stability of complexes. These interactions differ from covalent bonding in that they do not involve sharing of electrons.
Four types of noncovalent interactions are hydrogen bonds, van der Waals forces, ionic bonds, and hydrophobic interactions.
Hydrogen bonding results from the electrostatic attraction of a hydrogen atom covalently bonded to a strong-electronegative atom like oxygen,...
Four types of noncovalent interactions are hydrogen bonds, van der Waals forces, ionic bonds, and hydrophobic interactions.
Hydrogen bonding results from the electrostatic attraction of a hydrogen atom covalently bonded to a strong-electronegative atom like oxygen,...
50.3K
Thermal Sigmatropic Reactions: Overview
2.1K
Sigmatropic rearrangements are a class of pericyclic reactions in which a σ bond migrates from one part of a π system to another. These are intramolecular rearrangements where the total number of σ and π bonds remain unchanged.
Sigmatropic shifts are classified based on an order term [i, j ], where i and j indicate the number of atoms across which each end of the σ bond migrates. Below are examples of a [3,3] sigmatropic shift in...
Sigmatropic shifts are classified based on an order term [i, j ], where i and j indicate the number of atoms across which each end of the σ bond migrates. Below are examples of a [3,3] sigmatropic shift in...
2.1K
Atomic Absorption Spectroscopy: Lab
342
For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing...
Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing...
342
Atomic Absorption Spectroscopy: Atomization Methods
406
Atomic Absorption Spectroscopy (AAS) atomizes samples through flame atomization or electrothermal atomization. Flame atomization typically involves a nebulizer and spray chamber assembly to combine the sample with a fuel–oxidant mixture, creating a fine aerosol mist that enters a burner. Typically, the fuel and oxidant are combined in an approximately stoichiometric ratio. However, for atoms that are easily oxidized, a fuel-rich mixture may be more advantageous. Only about 5% of the...
406


