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在Obscuromonas (Euglenozoa:Trypanosomatidae:Blastocrithidiinae) 鞭毛动物中识别各种RNA病毒

Danyil Grybchuk^1,2, Arnau Galan¹, Donnamae Klocek¹

¹Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava 710 00, Czechia.

Virus evolution

|May 22, 2024

概括

这项研究探讨了Blastocrithidiinae中的病毒,这是一组单体类三体动物. 第一次在Obscuromonas中发现了米托病毒,它居住在细胞质中.

科学领域:

* 分子生物学和病毒学
* 寄生虫学和进化生物学

背景情况:

*三生虫是多样化的单细胞鞭毛虫,感染昆虫和/或脊椎动物.
* 单体三类虫携带各种RNA病毒,包括Narnaviridae,Totiviridae,Qinviridae,Leishbuviridae和类似于tombus的病毒.
*Blastocrithidiinae亚家族,包括奥布斯库罗莫纳斯和Blastocrithidia,是一个分离的和以前未被探索的群体.

研究的目的:

* 调查Blastocrithidiinae亚家族内的病毒景观.
* 用于描述 Obscuromonas 和 Blastocrithidia 的新型病毒感染.
* 为了记录三生虫中线粒病毒的存在和位置.

主要方法:

* 对Obscuromonas和Blastocrithidia分离物的基因组和转录组分析.
* 病毒序列鉴定和遗传学分析.
*对宿主基因组内的病毒元素进行生物信息分析.

主要成果:

* Obscuromonas分离的病毒来自Narnaviridae,Qinviridae和Mitoviridae家族的病毒.
* 首次在三生虫中报告了米托病毒,有证据表明细胞质复制.
* 布拉斯托克里蒂迪亚种类. 缺乏可检测的RNA病毒,但在B. triatomae中发现了一种与tombus类病毒相关的内源性病毒元素.

结论:

* Blastocrithidiinae亚家族拥有一个独特的病毒群体,包括奥布斯库罗莫纳斯的新型米托病毒感染.
* 在三生类动物中发现细胞质米托病毒扩大了我们对病毒复制策略的理解.
* 布拉斯托克里提迪亚的病毒接触包括过去感染的布类病毒,由内源性病毒元素表示.

关键词:

胚芽细胞菌体的发生.它们是Mitoviridae.纳尔纳维里亚类病毒昏暗的莫斯科病毒类似于的病毒.dsRNA病毒的病毒.

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