多糖Lycium barbarum重塑结肠炎症微环境,改善肠道健康状况
Rong Li1, Ping Yang1, Bowen Liu1
1School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, Sichuan, China.
巴巴多糖 (LBP) 可以通过恢复有益的细菌,如Dubosiella在性结肠炎 (UC) 鼠标模型中,改善肠道健康. 这种营养干预有助于重塑肠道.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 营养科学 营养科学
背景情况:
- 干扰的肠道微生物群与性结肠炎症 (UC) 中的炎症性结肠微环境有关.
- 现有的UC治疗有局限性,需要探索替代性肠道健康保护策略.
- 多糖被研究为潜在的有益营养物质,用于调节肠道微生物群和结肠炎症.
研究的目的:
- 研究Lycium barbarum多糖 (LBP) 在调节肠道微生物群和改善肠道健康方面的潜力.
- 评估LBP对炎症微环境和肠道屏障功能的影响,在硫酸 (DSS) 诱导的UC小鼠模型中.
主要方法:
- 在小鼠中使用硫酸 (DSS) 诱导UC.
- 使用Lycium barbarum多糖化物 (LBP) 评估其治疗效果.
- 对结肠组织结构,炎症指标,肠道屏障蛋白 (MUC-2,claudin-1,ZO-1) 和G蛋白结合受体5 (TGR5) 表达的分析.
- 用于肠道微生物群分析的16S rRNA测序和用于胆酸分析的液体染色学-双重质谱.
主要成果:
- 通过对MUC-2,Claudin-1和ZO-1进行上调,LBP显著改善了结肠组织完整性.
- LBP恢复了有益细菌的丰富性,特别是增加了占主导地位的Dubosiella,Akkermansia和Bifidobacterium属,同时减少了有害细菌.
- 在UC小鼠中,LBP促进了有益的胆汁酸 (甲醇酸和脱氧醇酸),通过TGR5上调来增强肠道屏障功能.
结论:
- 肠道微生物群平衡对于管理炎症性肠道微环境至关重要.
- 草甘 (Lycium barbarum polysaccharide,简称LBP) 在恢复肠道微生物群,特别是Dubosiella属,方面具有显著的潜力.
- 作为一种营养物质,LBP可能在肠道健康管理中发挥至关重要的作用,例如性结肠炎.
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