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Rapid Identification of Pathogens

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...

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Updated: Jul 4, 2026

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses

Published on: December 3, 2011

一种基于HPLC-SEC的囊性口腔炎病毒颗粒快速定量方法,以促进过程开发.

Adrian Schimek¹, Judy K M Ng¹, Ioannes Basbas¹

¹ViraTherapeutics GmbH, Bundesstraße 27, 6063 Rum, Austria.

Molecular therapy. Methods & clinical development

|May 22, 2024

概括

一种新的无标签方法使用尺寸排除色谱 (SEC) 和HPLC准确量化包裹病毒颗粒 (VPs). 这种高效的技术对于开发基于病毒的疗法和确保过程的一致性至关重要.

关键词:

在HPLC中使用HPLC.在VSV-GP中使用VSV.分析性的SEC证券交易所.膀性口腔炎病毒的病毒病毒颗粒量化定量化

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科学领域:

生物技术是生物技术.
分析化学分析化学
过程开发过程开发

背景情况:

准确的病毒粒子 (VP) 量化对于生产用于治疗应用的VP至关重要.
目前的方法可能缺乏效率或需要标签,阻碍了过程开发.

研究的目的:

开发和描述一种无标签的方法来量化包装VPs.
为VP生产建立可靠的工艺性能指标.

主要方法:

使用尺寸排除染色学 (SEC) 与高性能液态染色学 (HPLC) 相结合.
采用紫外线 (UV) 检测用于量化和多角度光散射 (MALS) 进行表征.
优化了移动阶段,并使用牛血清白蛋白 (BSA) 来稳定样本,实现>97%的回收.

主要成果:

证明了高精度,<1%的可重复性和<3%的中间精度变化.
建立了一个广泛的线性范围 (7.08 × 10^8至1.72 × 10^11 VP/mL),LOD为7.72 × 10^7 VP/mL,LLOQ为4.20 × 10^8 VP/mL.
已验证的适用于加工中的样品.

结论:

开发的SEC-HPLC方法为VP量化提供了一个精确,高效和无标签的方法.
该方法支持基于病毒的疗法的过程开发,包括瘤性病毒疗法,疫苗开发和基因疗法.
测试的速度和最小的实践时间使同一天的结果更容易用于过程监测.