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使用局部甲蓝以低成本扫描暗场微内镜成像核形态测量

Huayu Hou¹, Jennifer Carns¹, Richard A Schwarz¹

¹Rice University, Department of Bioengineering, Houston, Texas, United States.

Journal of biomedical optics

|May 22, 2024

概括

这项研究引入了一种新的光纤微内镜系统,使用甲蓝 (MB) 进行体内核形态测量. 该系统可实现实时,非侵入性可视化,用于早期癌症检测.

科学领域:

生物医学光学生物医学光学
医学成像医学成像
癌症诊断癌症诊断癌症诊断

背景情况:

光纤微内镜为早期癌症检测提供了上皮细胞核形态的非侵入性可视化.
临床应用受阻于缺乏合适的活体局部对比剂.

研究的目的:

通过使用新型光纤微内镜与局部甲蓝 (MB) 来评估体内核形态学成像.
MB是一种FDA批准的染料,用于胃肠染色镜检查.

主要方法:

一个低成本,高分辨率的微内镜利用扫描暗场成像与可编程照明和滚动快门.
通过对动物标本的ex vivo成像和对健康志愿者的in vivo成像进行验证.

主要成果:

扫描暗场成像显著减少了镜面反射,并改善了上皮细胞核的分辨率和对比度.
使用MB染色的图像质量与使用proflavine染色的光成像质量相当.

结论:

开发的系统可以实时对核模式进行显微镜评估.
这种方法有可能成为早期癌症检测的强大非侵入性工具.

关键词:

癌症检测癌症检测纤维光学微内镜的使用甲基烯蓝色是什么?甲基烯蓝色是什么?扫描暗场扫描暗场扫描

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