封闭导致粘附细胞聚合物的内部流动
M S Yousafzai1,2, S Amiri2,3, Z G Sun2,4
1Department of Biomedical Engineering, Yale University , , CT 06511, USA.
Journal of the Royal Society, Interface
|May 22, 2024
概括
细胞迁移和组织对流是复杂的. 这项研究揭示了细胞聚合物如何在表面上扩散,从而产生内部循环,平衡向外的迁移与向内收缩的流动,以获得净细胞运动.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 组织工程是组织工程.
背景情况:
- 介质细胞迁移涉及F-actin动力学和actomyosin收缩,影响力传输到细胞外基质.
- 由表面张力和压力梯度驱动的组织尺度对流也对细胞总体运动有所贡献.
- 单个细胞迁移和组织对流之间的协调仍然不太清楚.
研究的目的:
- 研究细胞迁移和对流是如何相互作用的,以确定细胞组合的净运动.
- 探索细胞聚合物在符合基质的粘合微模式上传播的动态.
主要方法:
- 研究了细胞聚合物在合规基板上的粘合微模式上的扩散.
- 观察到细胞单层扩张,突起稳定,收缩和脱落.
- 模拟细胞聚合物作为受限的活性滴.
主要成果:
- 细胞聚合物最初向外扩散,但无法稳定粘合边界之外的突起,导致收缩和脱落.
- 脱离的细胞表现出向上和向后的运动,创造大量的对流流向聚合物中心.
- 一个循环过程在向外突出的迁移和向内收缩的流动之间建立了平衡,从而产生了内部循环.
结论:
- 表面张力驱动的流动,向外的单层迁移和质量保存之间的相互作用驱动细胞聚合物的内部循环.
- 这种内部循环代表了表面迁移和逆向流动之间的平衡状态.
- 了解这些动态对于理解组织形态发生和集体细胞行为至关重要.
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