阴离子多糖化合物与内皮细胞表面结合 细胞外矩阵 涉及肝硫酸盐
Lu Fu1, Claire A Bridges1, Ha Na Kim2
1Graduate School of Biomedical Engineering, University of New South Wales, Sydney, NSW 2052, Australia.
Biomacromolecules
|May 22, 2024
概括
诸如多乙烯,乙烯) 葡萄糖胺 (PAAG) 这样的阴阳性多糖化物表现出强烈的结合于内皮糖体. 这种与肝素硫酸盐 (HS) 的相互作用影响了药物输送系统的设计.
科学领域:
- 生物材料科学 生物材料科学
- 细胞生物学 细胞生物学
- 药物输送系统 药物输送系统
背景情况:
- 阴离子多糖被探索用于血液中药物输送,但临床翻译是有限的.
- 内皮糖,一个阳离子细胞外基质,影响与带电分子的相互作用.
- 了解多糖与葡萄糖糖的相互作用对于有效的药物输送至关重要.
研究的目的:
- 为了研究充电多糖和内皮糖体之间的相互作用.
- 为了确定多糖质电荷如何影响与糖及其成分的关联.
- 阐明相互作用的机制及其对药物输送的功能后果.
主要方法:
- 聚乙烯,阿基尼尔) 葡萄糖胺 (PAAG),德克斯和氨酸与体外葡萄糖模型的相关性比较.
- 评估聚糖在糖中与硫酸乙 (HS) 的结合.
- 关于PAAG-HS相互作用的机制研究,包括基于电荷的关联和保护免受降解.
主要成果:
- PAAG与内皮糖核糖体的关联性最高,其次是中性德克斯和阳离子氨酸.
- PAAG 特别结合在糖中的肝硫酸盐 (HS),促进细胞内积累.
- 观察到多糖与HS的电荷依赖性关联.
- PAAG与HS结合是通过凝结发生的,并保护HS免受降解.
结论:
- 聚糖体电荷是与内皮糖体相互作用的关键决定因素.
- PAAG对HS的特定结合为增强细胞吸收和潜在的药物递送应用提供了一种机制.
- 了解这些相互作用可以指导改进的基于多糖的药物递送系统的设计.
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