相关实验视频

Updated: Jun 25, 2025

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry

Published on: October 2, 2016

在通用DNA条形码区域的商用kratom产品的序列变化

Kari Graham¹, Cesar Cantu¹, Rachel Houston¹

¹Department of Forensic Science, College of Criminal Justice, Sam Houston State University, Huntsville, Texas, USA.

Journal of forensic sciences

|May 22, 2024

概括

DNA 条形码提供了一个新的法医工具,用于跟踪 kratom (Mitragyna speciosa) 产品. 这种方法有助于识别商业kratom的变化,有助于对其非法分销的执法.

科学领域:

法医科学法医科学法医科学
植物分子生物学植物分子生物学
药理学是指药理学,即药理学是指药理学.

背景情况:

米特拉基纳特种 (kratom) 是一种用于缓解情绪和缓解疼痛的麻醉植物,作为"合法高"和阿片类替代品销售.
越来越多的过量服用导致了DEA和FDA的警告,但kratom仍然是联邦法律,使执法复杂化.
目前的法医方法,如形态学或化学分析,由于粉末样品和可变的化物含量,不足以归因来源.

研究的目的:

开发和评估一种DNA条形编码方法,用于评估商业kratom产品中的序列变异.
为了确定核 (ITS) 和 хлоропласт (matK,rbcL,trnH-psbA) 条形码区域的实用性,用于卡拉的识别.
探索DNA条形码的潜力,作为一个法医工具,用于Kratom可追溯性和执法.

主要方法:

从15种商业 kratom 产品中提取了DNA.
四个条形码区域被放大和测序:一个核 (ITS) 和三个叶绿体 (matK,rbcL,trnH-psbA).
在样本中分析了序列变化和多态性.

主要成果:

在分析的条形码区域内发现了新的多态.
内部转录间隔器 (ITS) 区域在样本中表现出最高的序列变化.
观察到有限的单元型多样性,在四个条形码区域中只确定了两个单元型.

结论:

DNA条形码显示出作为法医工具的承诺,用于Kratom的可追溯性和对非法分销的执法.
发现的多形态和ITS区域变异对于区分kratom样本非常有价值.
为了提高该方法的有效性,需要进一步开发和扩展M. speciosa DNA 序列数据库.

关键词:

米特拉基纳特异性植物 DNA 条形码编码测试DNA的类型.法医植物科学法医植物科学法医科学法医科学法医科学克拉托姆 (Kratom) 是一种标识物种标识物种标识物种

更多相关视频

Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification

Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification

Published on: November 1, 2024

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Published on: March 8, 2018

相关概念视频

Evolutionary Relationships through Genome Comparisons

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...

Gene Evolution - Fast or Slow?

Gene Evolution - Fast or Slow?

The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...