低密度颗粒细胞和中性粒细胞细胞外陷的形成在不完全的系统性红血狼中增加

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Svenja Henning¹, Tobias Reimers¹, Wayel Abdulahad¹

¹Department of Rheumatology and Clinical Immunology, University of Groningen, University Medical Centre Groningen, Groningen, The Netherlands.

Rheumatology (Oxford, England)

|May 22, 2024

概括

中性粒细胞功能障碍,包括低密度粒细胞 (LDGs) 和中性粒细胞细胞外陷 (NETs) 的增加,存在于早期系统性红斑狼 (SLE) 和不完整的SLE (iSLE). 这种功能障碍可能会导致新生SLE的自身抗原暴露和自身活性.

科学领域:

免疫学免疫学免疫学
类风湿病学类风湿病学
细胞生物学细胞生物学

背景情况:

系统性红斑狼 (SLE) 是一种复杂的自身免疫性疾病.
中性粒细胞功能障碍和中性粒细胞细胞外陷 (NETs) 涉及到SLE的发病.
SLE的早期阶段,包括不完整的SLE (iSLE),需要进一步调查中性粒细胞的参与.

研究的目的:

在患有iSLE和SLE的患者中研究低密度粒细胞 (LDG) 的比例.
评估在ISLE和SLE患者中循环的血NET和血清诱导的NET形成.
探索NET标志物与疾病特征之间的潜在相关性.

主要方法:

横截面研究测量LDGs (CD14lowCD15+) 通过流动细胞计在周围血液单核细胞 (PBMCs).
使用ELISA测定髓氧化酶-DNA,氨酸基因素H3和弹性酶-DNA复合物的循环血NET的量化.
通过将健康的中性粒细胞与患者或对照血清进行化,然后进行光显微镜,评估血清诱导的NET形成.

主要成果:

与健康对照人群相比,在iSLE和SLE患者中观察到高比例的LDG和循环血NET.
氧化 (HCQ) 治疗与较低的LDG相关.
来自iSLE和SLE患者的血清在健康的中性粒细胞中诱导了NET的形成;在iSLE中,骨髓氧化酶-DNA复合体与特定的B细胞子集相关.

结论:

中性粒细胞功能障碍,以增加的LDG和NET形成为特征,在早期的SLE (iSLE) 和已建立的SLE中明显.
这些中性粒细胞异常可能会在SLE的初期阶段导致自身抗原暴露和自身活性.
中性粒细胞功能障碍是SLE病变的潜在早期驱动因素,即使临床表现不同.

关键词:

一个小小的东西.没有完整的SLE.天生的免疫功能障碍.低密度的颗粒细胞.中性粒细胞外细胞陷

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